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Poster

Session: Session 2 Presenting Authors at Posters (Odds)

P-333 - Field dynamics of root endosphere microbiome assembly in paddy rice under fertilizer-free cultivation

Wednesday, July 16, 2025
13:30 - 15:15  
Location: Confex Hall 3
Plant microbiota functions and intermicrobial interactions
Plants often form beneficial interactions with microbial communities in response to nutrient deficiency. However, the processes governing microbial assembly and the underlying mechanisms, particularly in crops adapting to field conditions, remain poorly understood. In this study, we investigated root microbial communities in rice cultivated over four seasons in a high-yield paddy field that has been free of fertilization and pesticide use for at least 70 years. Using 16S rRNA amplicon sequencing, we analyzed the roots of three Japonica cultivars grown in both this nutrient-poor field and a neighboring, conventionally fertilized field. Our results suggest that fertilization regime and plant developmental stage had a greater impact on microbiome assembly than cultivar variation. Furthermore, a machine learning model and metagenomic analysis of nitrogenase genes indicate enhanced nitrogen fixation activity in the unfertilized field-grown roots, highlighting a potential role of the diazotrophic, iron-reducing bacterium Telmatospirillum. These findings provide valuable insights into soil nutrient-dependent assembly of the rice root microbiome in paddy fields, where mycorrhizal symbiosis is not effectively established, contributing to the development of sustainable agricultural practices.