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Poster

Session: Session 2 Presenting Authors at Posters (Odds)

P-127 - Identification Blumeria hordei AVRA effectors targets using proximity-dependent protein labelling in barley

Wednesday, July 16, 2025
13:30 - 15:15  
Location: Confex Hall 3
Effector Interference Mechanisms

    Presenting Author(s)

  • WS

    Wei Shi

    PostDoc
    Institute for Plant Sciences, University of Cologne
    Cologne, Nordrhein-Westfalen, Germany

    • Author(s)

  • MB

    Merle Bilstein-Schloemer

    PhD student
    Institute for Plant Sciences, University of Cologne
    Cologne, Nordrhein-Westfalen, Germany

  • SS

    Sara Stolze

    Max Planck Institute for Plant Breeding Research, Proteomics Group
    Koeln, Nordrhein-Westfalen, Germany

  • HN

    Hirofumi Nakagami

    Group leader
    Max Planck Institute for Plant Breeding Research
    Koeln, Nordrhein-Westfalen, Germany

  • IS

    Isabel Saur

    Group leader
    University of Cologne, CEPLAS
    Cologne, Nordrhein-Westfalen, Germany

Blumeria hordei (Bh) causes the powdery mildew disease in barley. The Bh AVRA effectors (AVRAs) are recognized by NLR immune receptors encoded at Mildew locus a (Mla) in resistant barley lines. The maintenance of AVRA effectors in the Bh population despite their detection by MLA receptors suggests that the effector’s intrinsic function is essential for Bh virulence. However, these virulence functions remain largely unknown. We applied a combination of in vivo proximity-dependent protein labelling and co-immunoprecipitation using AVRA-expressing stable transgenic barley lines to isolate the host targets of AVRA effectors and get insight into AVRA virulence functions. We identified multiple high-quality candidate interactors of AVRA1, AVRA7, AVRA9, AVRA10 and AVRA13. Despite structural conservation of these AVRAs, the interactome of individual AVRAs do not overlap suggestion different functions in promoting Bh virulence. We identified the barley Strubbelig Receptor Family 3 (HvSRF3) receptor-like kinase as specific interactor of AVRA13. AVRA13 interacts with the cytoplasmic domains of only certain barley SRF3 isoforms and influences SRF3 plasma membrane depletion. Together, our data suggests that a) structurally related AVRA effectors have distinct intrinsic functions and that b) SRF3 activity modulates plant immunity towards powdery mildew and potentially other pathogens.