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Poster

Session: Session 2 Presenting Authors at Posters (Odds)

P-133 - Insights into the Verticillium dahliae effector repertoire during xylem infection of potato

Wednesday, July 16, 2025
13:30 - 15:15  
Location: Confex Hall 3
Effector Interference Mechanisms

    Presenting Author(s)

  • LF

    Luigi Fiorentino

    Wageningen University and Research
    Wageningen, Gelderland, Netherlands

    • Author(s)

  • DW

    Doret Wouters

    Plant Breeding, Wageningen University and Research
    Wageningen, Gelderland, Netherlands

  • BS

    Barbara Schrammel

    PhD
    Plant Breeding, Wageningen University and Research
    Wageningen, Gelderland, Netherlands

  • JW

    Jasmin Wiedmer

    Plant Breeding, Wageningen University, The Netherlands.
    Wageningen, NETHERLANDS

  • PW

    Pieter J. Wolters

    Plant Breeding, Wageningen University and Research
    Wageningen, Gelderland, Netherlands

  • RG

    Richard G. F. Visser

    Plant Breeding, Wageningen University, The Netherlands.
    Wageningen, Gelderland, Netherlands

  • VV

    Vivianne Vleeshouwers

    Wageningen University & Research
    Wageningen, Gelderland, Netherlands

Verticillium wilt is a major disease in potato (Solanum tuberosum) and other Solanaceae caused by the soil-borne fungus Verticillium dahliae. It is well established that V. dahliae possesses a variegate effector repertoire that facilitates manipulation of the soil microbiome and host colonization. Previous studies, primarily in cotton and tomato, have identified various effectors that enhance Verticillium pathogenicity, suppress plant defence pathways and promote plant senescence. In this study, we used a new approach to predict V. dahliae effectors that are expressed during infection of potato. To achieve this, V. dahliae mycelium was directly sprayed with xylem sap extracted from a susceptible potato cultivar. RNA was extracted from the mycelium and subjected to RNA-Seq analysis. We established an effector mining pipeline and discovered a large set of V. dahliae effectors. From this set, we selected 98 effectors for further study and based on the presence of a signal peptide and predicted secretion pathway, we categorized them as either canonical secreted effectors or unconventionally secreted effectors. These effectors are currently being functionally screened for causing defense responses mediated by immune receptors in resistant wild Solanum accessions. Our ultimate goal is to identify these receptors that can be introduced into potato cultivars to enhance resistance to Verticillium wilt.