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Poster

Session: Session 2 Presenting Authors at Posters (Odds)

P-255 - Plant defence chemicals control the root endophytism in Arabidopsis thaliana

Wednesday, July 16, 2025
13:30 - 15:15  
Location: Confex Hall 3
Nutrient exchange & role of metabolites in plant-microbe interactions

    Presenting Author(s)

  • SB

    SUBHANKAR BERA, PhD

    Postdoc
    Malopolska Center of Biotechnology, Jagiellonian University
    Krakow, Malopolskie, Poland

    • Author(s)

  • AB

    Arpan Kumar Basak

    Max Planck Institute for Plant Breeding Research
    Köln, Nordrhein-Westfalen, Germany

  • MM

    Mohamadreza Mirzaei

    Doctoral student
    Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
    Kraków, Malopolskie, Poland

  • MP

    Mariola Pislewska-Bednarek

    Institute of Bioorganic Chemistry, Polish Academy of Sciences
    Poznan, Wielkopolskie, Poland

  • SG

    Swati Sweta. Ghadei

    Masters student
    Department of Medical Biotechnology, Jagiellonian University, Krakow, Poland
    Kraków, Malopolskie, Poland

  • AP

    Anna Piasecka

    Institute of Bioorganic Chemistry, Polish Academy of Sciences
    Poznań, Wielkopolskie, Poland

  • RW

    Rafał Ważny

    Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland
    Krakow, Malopolskie, Poland

  • PR

    Piotr Rozpądek

    Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland
    Krakow, Mazowieckie, Poland

  • PB

    Pawel Bednarek

    Institute of Bioorganic Chemistry, Polish Academy of Sciences
    Poznan, Wielkopolskie, Poland

  • KY

    Kenji Yamada

    Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland
    Kraków, Malopolskie, Poland

Brassicaceae plants have developed robust defence mechanisms through unique secondary metabolites, such as aliphatic glucosinolates (AG), indole glucosinolates (IG) and camalexin. Previous studies indicate that tryptophan-derived metabolites influence Arabidopsis thaliana root microbiota assembly. However, the precise interplay between the chemical defence and root-endophyte behaviour remains unclear. We explore how plants and endophytes change their behaviour in the absence of host defence chemicals using an endophyte Phomophsis columnaris and A. thaliana. We found that P. columnaris adversely affects the growth of cyp79b2,b3,myb28,29 quadruple mutant (qKO) that lacks AG, IG and camalexin. In this interaction, activation of PDF genes was observed in the host plants, whereas the fungi extensively activated carbohydrate-active and plant cell wall degrading enzyme genes. P. columnaris did not show an adverse effect on AG-deficient myb28,29 or IG-deficient myb34,51,122 mutants. The hyper-accumulation of camalexin was observed in myb28,29 and myb34,51,122 mutants, suggesting that the mutants prevent fungi overload by enhancing camalexin biosynthesis to compensate for the absence of other defence chemicals. These findings indicate that the host plants reprogram transcriptional profiling to overcome the defect of defensive metabolites, which is the combination of AG, IG and camalexin.