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Poster

Session: Session 1 Presenting Authors at Posters (Evens)

P-264 - Ty-2 resistance-breakdown by geminivirus of the Tomato yellow leaf curl virus disease (TYLCD) complex

Tuesday, July 15, 2025
09:55 - 11:30  
Location: Confex Hall 3
Plant-virus Interactions (incl. Viromes, Evolution & Vectors)

Isolates of geminiviruses associated with the tomato yellow leaf curl disease (TYLCD), such as Tomato yellow leaf curl virus (TYLCV), cause severe damage to tomato (Solanum lycopersicum) crops worlwide. The Ty-2 gene from S. habrochaites is effective against the most widespread strain of TYLCV (officially the type strain and called Israel, IL, strain) although can be overcome by isolates of other TYCLV strains such as the Mild (Mld) strain. Ty-2 is a “nucleotide-binding leucine-rich repeat (NLR)” that is constitutively expressed in the resistant lines and is not regulated upon TYLCV infection. Several analyses have determined that the C4 protein is the viral determinant to overcome Ty-2 derived resistance. The aim of this study is to determine the mechanisms involved in Ty-2-mediated differential resistance to several viruses of the TYLCD complex. Using recombinant virus versions for the C4 gene from TYLCV-IL and TYLCV-Mld, we have identified a region of the protein that contains the RCC1-like domain (RLD), that is involved in the Ty-2 resistance breakdown and that is present in C4 -IL but not C4-Mld. Two-point mutations in this domain that disrupted the interaction between C4-IL and RLD, were sufficient to overcome Ty-2-mediated resistance, suggesting a link between a NLR-mediated resistance and RLD, a protein involved in the determination of cell polarity. The subcellular localization of these three proteins will also be discussed and presented.