P-376 - Molecular characterization of the immune response of Arabidopsis thaliana to cell wall-derived glycans

Tuesday, July 15, 2025

09:55 - 11:30

Location: Confex Hall 3

Subcellular dynamics and signaling in host-microbe interactions

Presenting Author(s)

KC

Klara Culjak

Centro de Biotecnología y Genómica de Plantas (CBGP), Centro Severo Ochoa, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA/CSIC)
Madrid, Madrid, SPAIN

Author(s)

HG

Hiba Ghrissi

PhD student
Centro de Biotecnología y Genómica de Plantas (CBGP), Centro Severo Ochoa, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA/CSIC)
Madrid, Madrid, Spain
DB

Diego José Berlanga

Centro de Biotecnología y Genómica de Plantas (CBGP), Centro Severo Ochoa, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA/CSIC)
Madrid, Madrid, Spain
MM

Marina Martín-Dacal

Centro de Biotecnología y Genómica de Plantas (CBGP), Centro Severo Ochoa, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA/CSIC)
Madrid, Madrid, Spain
VK

Varun Kumar

Mahindra University
Hyderabad, Telangana, India
PF

Patricia Fernández-Calvo

MBG CSIC
Santiago de Compostela, Galicia, Spain
JJ

José María Jiménez Gómez

Researcher CSIC-INIA
Centro de Biotecnología y Genómica de Plantas (CBGP), Centro Severo Ochoa, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA/CSIC)
Madrid, Madrid, Spain
MT

Miguel Ángel Torres

Associate Professor
Centro de Biotecnología y Genómica de Plantas (CBGP), Centro Severo Ochoa, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA/CSIC)
Madrid, Madrid, Spain
AM

Antonio Molina

Full Professor
Centro de Biotecnología y Genómica de Plantas (CBGP), Centro Severo Ochoa, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA/CSIC)
Madrid, Madrid, Spain
LJ

Lucia Jorda, PhD (she/her/hers)

Associate Professor
Centro de Biotecnología y Genómica de Plantas (CBGP), Centro Severo Ochoa, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA/CSIC)
Pozuelo de Alarcon, Madrid, Spain

Plants have evolved a complex immune system to resist pathogens. One layer of this system is pattern-triggered immunity (PTI), activated when extracellular ectodomains (ECDs) of plant pattern recognition receptors (PRRs) detect microbe-associated molecular patterns, such as carbohydrates from microbial outer layers, or damage-associated molecular patterns, like glycans from plant cell walls. We conducted a genetic screen to identify Arabidopsis thaliana mutants impaired in glycan perception (igp), and we discovered a family of receptor kinases (IGP1/CORK1, IGP3, and IGP4) with leucine-rich repeats and malectin domains (LRR-MAL RK) in their ECDs. These RKs are essential for PTI responses triggered by cellulose-derived oligosaccharides, such as the CEL3 trisaccharide, as well as by mixed-linkage β-1,3/1,4-glucans (MLGs). The ECD of IGP1, but not IGP4, binds CEL3, confirming IGP1’s role as a PRR for cellulose-derived oligosaccharides and suggesting that IGP4 functions as a co-receptor. Genetic complementation analyses of additional igp mutants (igp9, igp16, igp17, igp18, igp19) revealed novel IGP1 and IGP4 alleles, highlighting the importance of these RKs in glycan-mediated PTI. Additional igp mutants selected in the screen were impaired in novel molecular components required for CEL3/MLG43-mediated PTI. The importance of these novel IGP proteins in PTI and disease resistance, as well as their potential role in designing sustainable crop protection solutions, will be discussed.