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Poster

Session: Session 2 Presenting Authors at Posters (Odds)

P-083 - Unraveling the Genetic Basis of Sr43-Mediated Stem Rust Resistance in Wheat

Wednesday, July 16, 2025
13:30 - 15:15  
Location: Confex Hall 3
Crop resistance genetics and genomics

    Presenting Author(s)

  • YW

    Yan L. Wang

    Dr.
    King Abdullah University of Science and Technology (KAUST)
    Thuwal, Makkah, Saudi Arabia

    • Author(s)

  • KG

    Karthick Gajendiran

    King Abdullah University of Science and Technology (KAUST)
    Thuwal, Makkah, Saudi Arabia

  • RC

    Renjie Chen

    Dr.
    King Abdullah University of Science and Technology (KAUST)
    Thuwal, Makkah, Saudi Arabia

  • OP

    Oliver Powell

    King Abdullah University of Science and Technology (KAUST)
    Thuwal, SAUDI ARABIA

  • GY

    Guotai Yu

    Dr.
    King Abdullah University of Science and Technology (KAUST)
    Thuwal, Makkah, Saudi Arabia

  • TN

    Thorsten Nuernberger

    University of Tü​bingen
    Tuebingen, Baden-Wurttemberg, Germany

  • WB

    Willem Boshoff

    Prof. Dr.
    University of the Free State, Bloemfontein, South Africa
    Bloemfontein, Free State, South Africa

  • BW

    Brande Wulff

    Prof. Dr.
    King Abdullah University of Science and Technology (KAUST)
    Thuwal, Makkah, Saudi Arabia

Kinase fusion proteins (KFPs) are an emerging group of resistance proteins identified in Triticeae. In our previous work, we cloned the stem rust (Puccinia graminis f. sp. tritici) resistance gene Sr43 from Thinopyrum obtusiflorum, which encodes a KFP containing a kinase domain and two domains of unknown function. In the current study, we identified four Sr43 extragenic EMS mutants—1850h, 1575f, 187g, and 43f—that have lost resistance to the stem rust isolate UK01. Following backcrossing, we discovered that the BC1F2 populations of 1850h, 1575f, and 43f exhibited a 3:1 resistance-to-susceptible segregation ratio after inoculation with race PTKST. The F1 generation from a complementary cross between 1850h and 1575f showed a susceptible phenotype. Currently, we are using bulk segregant analysis (BSA)-sequencing to identify the Sr43 suppressor(s). This will allow us to further study the mechanism of Sr43-mediated resistance in wheat, ultimately contributing to crop breeding efforts against stem rust.