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Poster

Session: Session 2 Presenting Authors at Posters (Evens)

P-432 - Cell-Type-Specific Autophagy Modulates Pseudomonas syringae Infection Dynamics in Arabidopsis

Thursday, July 17, 2025
13:30 - 15:15  
Location: Confex Hall 3
Spatial & temporal resolution of the interaction interface

    Presenting Author(s)

  • SZ

    Shanshuo Zhu

    Faculty of Biology and Biotechnology, Ruhr-University of Bochum
    Bochum, Nordrhein-Westfalen, Germany

    • Author(s)

  • GL

    Gautier Langin

    Faculty of Biology and Biotechnology, Ruhr-University of Bochum
    Bochum, Nordrhein-Westfalen, Germany

  • PS

    Pavel Solansky

    Center for Plant Molecular Biology, Developmental Genetics, University of Tübingen
    Tübingen, Baden-Wurttemberg, Germany

  • TD

    Tom Denyer

    Center for Plant Molecular Biology, Developmental Genetics, University of Tübingen
    Tübingen, Baden-Wurttemberg, Germany

  • MT

    Marja C. P. Timmermans

    Center for Plant Molecular Biology, Developmental Genetics, University of Tübingen
    Tübingen, Baden-Wurttemberg, Germany

  • SU

    Suayib Uestuen

    Ruhr-University Bochum
    Bochum, Nordrhein-Westfalen, Germany

Autophagy, a conserved proteolytic pathway, plays a critical role in plant immunity. Recent studies have highlighted the importance of autophagy in both host cell defense mechanisms and bacterial interactions. However, the cell-type-specific modulation of this pathway by bacteria remains poorly understood. In this study, we employed single-cell transcriptomics to analyze cell-type-specific responses to Pseudomonas syringae pv. tomato DC3000 (Pst) infection in wild-type Arabidopsis thaliana Col-0 and the autophagy-deficient atg5 mutant. Our findings reveal that Pst significantly activates autophagy in guard cells during early infection, promoting stomatal opening to facilitate bacterial entry. Mechanistically, autophagy targets ABA receptors for degradation, thereby suppressing ABA signaling to enable stomatal aperture. Beyond its role in innate immunity, the atg5 mutant demonstrates enhanced immune responses in mesophyll cells and exhibits stress priming, highlighting the diverse roles of autophagy across tissues. This study provides novel insights into the microbe-modulated autophagy response at single-cell resolution and underscores the cell-type-specific functions of autophagy in plant immunity.