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Poster

Session: Session 2 Presenting Authors at Posters (Evens)

P-318 - Directed selection of suppressive rhizosphere microbiome community helps to overcome the growth-defence trade-off

Thursday, July 17, 2025
13:30 - 15:15  
Location: Confex Hall 3
Plant microbiota functions and intermicrobial interactions

    Presenting Author(s)

  • TK

    Tetiana Kalachova, PhD

    researcher
    Institute of Experimental Botany Czech Academy of Sciences
    Prague, Czech Republic

    • Author(s)

  • BJ

    Barbora Jindřichová

    Institute of Experimental Botany Czech Academy of Sciences
    Prague, Hlavni mesto Praha, Czech Republic

  • MB

    Manuel Blouin

    Universite Bourgogne Franche-Comte
    Dijon, Bourgogne, France

  • LB

    Lenka Burketová

    Institute of Experimental Botany Czech Academy of Sciences
    Prague, Hlavni mesto Praha, Czech Republic

  • ER

    Eric Ruelland

    Université de Technologie de Compiègne
    Compiegne, Picardie, France

  • RP

    Ruben Puga-Freitas

    Université Paris Est Creteil
    Creteil, Ile-de-France, France

The rhizosphere microbiome contributes to nutrient acquisition, protection against biotic and abiotic stresses and, ultimately, development and physiology of plants. We followed the microbial dynamics in the soil of Arabidopsis thaliana infected with the foliar pathogen Pseudomonas syringae pv. tomato DC3000 (Pst). Soil microbial communities were selected using directed or indirected (i.e. natural) selection approaches. To do so, plants were iteratively cultivated on a pasteurized soil inoculated with the soil microbial community of the previous iteration isolated from the rhizosphere of plants infected with Pst (pst-line) or not (mock-lines). Out of the infected plants, two groups were designated based on disease manifestation in leaves: suppressive and conductive line. Modification of microbial communities was assessed along with plant fitness and transcript abundance of stress related genes. At the tenth and eleventh iterations, we observed a reduction in disease severity in pst-lines as compared to mock-lines, accompanied with the smaller rosette size in the line deriving from the natural selection, but not in the directly selected suppressive line. These changes were associated with altered transcription of genes belonging to signaling pathways of salicylic, jasmonic and abscisic acid, and a shift in soil bacterial (but not fungal) composition. This supports the concept of targeting plant-soil feedbacks to enhance soil suppressiveness against foliar pathogens.