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Poster

Session: Session 2 Presenting Authors at Posters (Evens)

P-208 - Interspecies metabolic engineering of Trp-derived metabolites for their function in plant immunity

Thursday, July 17, 2025
13:30 - 15:15  
Location: Confex Hall 3
Engineering plant-microbe traits

    Author(s)

  • GS

    Gopal Singh

    Institute of Bioorganic Chemistry, Polish Academy of Sciences
    Poznan, Wielkopolskie, Poland

  • HA

    Himani Agrawal

    Institute of Bioorganic Chemistry, Polish Academy of Sciences
    Poznań, Wielkopolskie, Poland

  • AP

    Anna Piasecka

    Institute of Bioorganic Chemistry, Polish Academy of Sciences
    Poznań, Wielkopolskie, Poland

  • VL

    Veeresh Lokesh

    Institute of Bioorganic Chemistry, Polish Academy of Sciences
    Poznań, Wielkopolskie, Poland

  • SB

    Sylwia Bugaj

    Institute of Bioorganic Chemistry, Polish Academy of Sciences
    Poznań, Wielkopolskie, Poland

  • MP

    Mariola Pislewska-Bednarek

    Institute of Bioorganic Chemistry, Polish Academy of Sciences
    Poznan, Wielkopolskie, Poland

    • Presenting Author(s)

  • PB

    Pawel Bednarek

    Institute of Bioorganic Chemistry, Polish Academy of Sciences
    Poznan, Wielkopolskie, Poland

Trp-derived indolic glucosinolates and phytoalexins are among well-studied specialized metabolites with roles in immunity of Brassicaceae species. Arabidopsis thaliana mutants deficient in particular pathway enzymes helped to reveal specific functions of particular Trp-derived metabolites in pre-invasive and post-invasive phases of fungal infection. However, it is still unclear if specific functions of these compounds are defined by their spatiotemporal accumulation patterns or by their unique biological activities. Several members of Poaceae family, including Brachypodium distachyon, have been reported to produce serotonin as Trp-derived metabolite in response to infection. However, serotonin involvement in plant immunity remains ambiguous. To explore this, we have engineered serotonin biosynthetic pathway in A. thaliana mutant unable to produce endogenous Trp-derived metabolites. To address the of impact spatiotemporal accumulation patterns on metabolite function we opted to use A. thaliana native promoters controlling Trp-metabolism during pre- and post-invasive phase of immune responses. Metabolomic analysis confirmed the presence of tryptamine and serotonin together with several novel metabolites in transgenic A. thaliana lines. Overall, we have demonstrated the successful application of advanced synthetic biology and metabolic engineering tools in stable engineering of multistep biosynthetic pathways under native conditions.