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Poster

Session: Session 1 Presenting Authors at Posters (Odds)

P-045 - Identifying the LRR-RLP type immune receptors that recognize pathogen-derived molecules in plants

Monday, July 14, 2025
13:30 - 15:15  
Location: Confex Hall 3
Extracellular & Intracellular non-self recognition & signaling
Plants recognize microbial molecules, known as Microbe-Associated Molecular Patterns (MAMPs), through cell-surface immune receptors called pattern recognition receptors (PRRs). Among these PRRs, leucine-rich repeat receptor-like protein (LRR-RLP) families play central roles for recognizing peptide- or proteinaceous MAMPs. LRR-RLPs genes have undergone lineage-specific expansion in higher plants, suggesting an evolutionary strategy to broaden recognition capabilities by expanding the immune receptor families. However, their molecular identities in non-model plants remain largely unexplored due to the lack of genetic tools for receptor identification. To identify LRR-RLP-type immune receptors in various plants, including non-model plants, we extracted over 11,000 LRR-RLP sequences from 350 publicly available plant and algae genomes. Since many of these receptors might be redundant and recognize the same ligand, we analyzed the conservation of amino acid residues in estimated ligand-binding regions to categorize PRRs into groups likely recognizing the same ligands. We then cloned representative PRR genes from each group and transiently expressed them in Nicotiana benthamiana to assess the activation in response to living pathogens, pathogen extracts, and MAMPs with unidentified receptors. Given that LRR-RLP type immune receptors can be transferred between species, the identification of novel PRRs could significantly advance the development of pathogen-resistant crops.