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Poster

Session: Session 1 Presenting Authors at Posters (Odds)

P-065 - Placing Poly (ADP-ribose) polymerases in the plant immune signalling network

Monday, July 14, 2025
13:30 - 15:15  
Location: Confex Hall 3
Extracellular & Intracellular non-self recognition & signaling

    Presenting Author(s)

  • RR

    Rajalakshmi Reghunath

    Leibniz Institute of Plant Biochemistry (IPB)
    Halle(Saale),Saxony-Anhalt, Germany, GERMANY

    • Author(s)

  • JZ

    Jörg Ziegler

    Leibniz Institute of Plant Biochemistry (IPB)
    Halle(Saale), Sachsen-Anhalt, Germany

  • GB

    Gerd Balcke

    Leibniz Institute of Plant Biochemistry
    Halle, Sachsen-Anhalt, Germany

  • SM

    Susanne Matschi

    Postdoc
    Leibniz-Institute of Plant Biochemistry
    Halle, Sachsen-Anhalt, Germany

  • DT

    Domenika Thieme

    Leibniz Institute of Plant Biochemistry
    Halle Saale, Sachsen-Anhalt, Germany

  • LW

    Lennart Wirthmüller

    Group Leader
    Leibniz Institute of Plant Biochemistry (IPB)
    Halle, Sachsen-Anhalt, Germany

Poly-ADP-ribosylation (PARylation) is a reversible post-translational protein modification. In PARylation, ‘writer’ enzymes of the Poly (ADP-ribose) polymerases (PARP) family repeatedly transfer ADP-ribose moieties from the co-substrate nicotinamide adenine dinucleotide (NAD+) onto target proteins, thereby catalysing the formation of long poly (ADP-ribose) chains. We report that an Arabidopsis thaliana parp1/2/3 higher-order mutant exhibits enhanced susceptibility to Botrytis cinerea. This elevated susceptibility towards a necrotrophic fungal pathogen correlates with higher transcript levels of salicylic acid-regulated defence genes in the mutant. Comparative metabolome profiling of the parp1/2/3 mutant and wild type plants in response to a PAMP stimulus suggest additional functions of PARPs in re-programming the phenylpropanoid branch of the defence metabolome. In order to place PARPs more precisely in the plant immunity network, we integrate proteomics data on PARP-interacting proteins and affinity enrichment of potential PARylated substrates. We will present on our current efforts to identify molecular links between PARPs and regulators of the defence transcriptome and metabolome.