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Poster

Session: Session 2 Presenting Authors at Posters (Odds)

P-197 - Editing of an antiviral host factor boosts plant growth and yield of plant viral vector-mediated heterologous protein expression

Wednesday, July 16, 2025
13:30 - 15:15  
Location: Confex Hall 3
Engineering plant-microbe traits

    Author(s)

  • ZF

    Zhu Fang

    Institute Of Plant Protection, Chinese Academy Of Agricultural Sciences
    Beijing, Beijing, China (People's Republic)

  • XZ

    Xinru Zhao

    Institute of Plant Protection, Chinese Academy of Agricultural Sciences
    Beijing, Beijing, China (People's Republic)

  • MD

    Min Du

    Institute of Plant Protection, Chinese Academy of Agricultural Sciences
    Beijing, Beijing, China (People's Republic)

  • XX

    Xinyi Xu

    Institute of Plant Protection, Chinese Academy of Agricultural Sciences
    Beijing, Beijing, China (People's Republic)

  • HZ

    Hui Zhou

    Institute of Plant Protection, Chinese Academy of Agricultural Sciences
    Beijing, Beijing, China (People's Republic)

  • WG

    Wei Guo

    Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences
    Beijing, Beijing, China (People's Republic)

  • XZ

    Xueping Zhou

    Institute of Plant Protection, Chinese Academy of Agricultural Sciences
    Beijing, Beijing, China (People's Republic)

    • Presenting Author(s)

  • XY

    Xiuling Yang (she/her/hers)

    Institute of Plant Protection, Chinese Academy of Agricultural Sciences
    Beijing, Beijing, China

Plant viruses have been modified as important tools to express heterologous proteins in plants, yet the yield of target proteins is often limited by plant antiviral defenses. In this study, we found that the mRNA level of a glycine-rich cell wall structural protein (NbGRP) was significantly upregulated in response to tomato brown rugose fruit virus (ToBRFV) infection. We show that NbGRP is an antiviral host factor by gain and loss-of function assays. We demonstrated that CRISPR/Cas9-based genome editing of NbGRP not only promotes plant growth but also significantly enhances the production of heterologous proteins mediated by three different plant viral vectors. Our study provides a promising strategy to optimize plant viral vector-based biofactories, with potential applications in biotechnology.