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Poster

Session: Session 2 Presenting Authors at Posters (Odds)

P-341 - Genetic structure and characterization of plant growth-promoting rhizobacteria in citrus citrumelo Swingle rootstock under different agricultural systems

Wednesday, July 16, 2025
13:30 - 15:15  
Location: Confex Hall 3
Plant microbiota functions and intermicrobial interactions

    Author(s)

  • NM

    Nathalia Mancine

    Agronomic Institute - IAC / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • GP

    Giovana Peruchi

    Agronomic Intitute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • AV

    Alessia Volpini

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • LM

    Lais Moura

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • HL

    Helena Lima

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

    • Presenting Author(s)

  • HC

    Helvecio Coletta-Filho

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

Environmental conditions and agricultural management shape microbial communities in the plant rhizosphere. While most research focuses on annual crops, little is known about perennial plants under different tillage systems and plant growth-promoting rhizobacteria (PGPR). This study examines bacterial communities in citrus roots under organic (OM) and conventional (CM) management to characterize microbial structures and develop synthetic communities for bio-inputs. Root and rhizosphere soil samples were cultured in four media, yielding 3776 isolates. Taxonomy was determined for 424 individuals by sequencing the V4-V5 region of the 16S rRNA gene using Illumina technology and classifying bacteria at the genus level via the amplicon sequence variant (ASV) strategy. About 80% of ASVs were shared between both systems. Among sequenced isolates, 41 different genera belonged to PGPR. In vitro tests identified 45 ASVs with plant growth-promoting activities, including phosphate solubilization, auxin (IAA) production, siderophore production, and nitrogen fixation. OM-ASVs showed higher siderophore production and phosphate solubilization, while CM-ASVs had greater IAA production and nitrogen fixation. From this, 25 ASVs were selected for in vivo tests. OM-ASVs accelerated seed germination, while CM-ASVs increased root volume. Further tests on citrus seedlings for growth and phosphate solubilization are ongoing.

Supported by Fapesp: (2020/14584-8 and 2023/01392-1)