P-051 - Hidden networks: A new molecular pathway regulating tomato immunity

Plants continuously need to integrate environmental and internal signals to adjust growth, development and metabolism to increase fitness in an everchanging environment. Integration of these cues relies on intricate signaling networks that govern the adaptive immunity programs essential to safeguard plant development and survival. Jasmonates and salicylic acid are essential signaling molecules in these networks. Distinct protein-protein interactions facilitate the formation of unique macromolecular complexes involved in the perception and transmission of these hormone signals. Therefore, we applied TurboID-based proximity labeling in tomato (Solanum lycopersicum) hairy roots with stress hormone signaling components as a bait. We identified TIFY8, a transcriptional regulator, as a common component across multiple Turbo-ID datasets, suggesting a role in defense regulation. Our interactome analysis revealed WRKY transcription factors and OBERON histone-binding proteins as TIFY8 interactors and potential downstream regulators of tomato immunity. Currently, we are conducting molecular assays and functional analyses in edited and transformed tomato plants to understand the mechanistic functions of TIFY8 and its interactors in tomato immunity. The findings of this project will improve our understanding of the biological mechanisms controlling plant immunity and may ultimately be translated into crop engineering programs focusing on enhanced plant resilience.