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Poster

Session: Session 2 Presenting Authors at Posters (Odds)

P-363 - New plasmids on the block: Tools for complex genetic modifications in prokaryotes

Wednesday, July 16, 2025
13:30 - 15:15  
Location: Confex Hall 3
Plant microbiota functions and intermicrobial interactions

    Author(s)

  • SF

    Sylvain Fochesato

    Reserach Engineer
    CEA
    St Paul lez Durance, Provence-Alpes-Cote d'Azur, France

  • MG

    Maxence Girardin

    PhD student
    CEA
    St Paul lez Durance, Provence-Alpes-Cote d'Azur, France

  • JD

    Julien Dou

    PhD student
    CEA
    St Paul lez Durance, Provence-Alpes-Cote d'Azur, France

  • SM

    Sylvestre Marillonnet

    Principal Investigator
    Leibniz Institute for Plant Biochemistry
    Halle an der Saale, Sachsen-Anhalt, Germany

    • Presenting Author(s)

  • JS

    Johannes Stuttmann

    Researcher
    CEA
    St Paul Lez Durance, Provence-Alpes-Cote d'Azur, France

Environmental bacteria including plant-associated microbiota hold broad potential for applications in agriculture and biotechnology. However, their genetic modification may be challenging, and researchers often rely on a limited number of well-established, but inconvenient plasmid tools for transformation. We developed a systematic, flexible and scalable framework for assembly of plasmids incorporating diverse replication origins, resistance markers and further components, based on Golden Gate assembly. Plasmids can subsequently be loaded with genetic cargoes relying on hierarchical DNA assembly following the Modular Cloning grammar, thus allowing assembly of complex genetic systems for different applications. We currently develop DNA modules for diverse genome editing applications, and test these in different prokaryotic systems. Our assembly platform and currently established applications will be showcased.