P-065 - Phylogenomic insights into NLR diversity and evolution in Caryophyllales

Wednesday, July 16, 2025

13:30 - 15:15

Location: Confex Hall 3

Crop resistance genetics and genomics

Presenting Author(s)

NL

Nutthalak Laksanavilat

Post-doctoral scientist
The Sainsbury Laboratory
Norwich, England, United Kingdom

Author(s)

AT

AmirAli Toghani (he/him/his)

PhD Student
The Sainsbury Laboratory
Norwich, England, United Kingdom
DL

Daniel Lüdke

Post-doctoral scientist
The Sainsbury Laboratory
Norwich, England, United Kingdom
TS

Toshiyuki Sakai

Laboratory of Crop Evolution, Graduate School of Agriculture, Kyoto University
Kyoto, Kyoto, Japan
HA

Hiroaki Adachi

Laboratory of Crop Evolution, Graduate School of Agriculture, Kyoto University
Kyoto, Kyoto, Japan
AB

A. Cristina Barragan

The Sainsbury Laboratory
Norwich, England, United Kingdom
SK

Sophien Kamoun

Group leader
The Sainsbury Laboratory
Norwich, England, United Kingdom

The order Caryophyllales encompasses a wide diversity of plant species, including crops and stress-adapted xerophytes, yet the composition of their immune receptor repertoire remains largely unexplored. To investigate resistance gene diversity in this lineage, we performed a phylogenomic analysis of 5,277 nucleotide-binding leucine-rich repeat (NLR) immune proteins across 29 Caryophyllales species. Our findings reveal substantial variation in NLR abundance and composition compared to well-studied plant orders like Solanales and Asterales, with an average of 181 NLRs per species. Notably, CC-NLRs (coiled-coil domain) and CC_G10-NLRs dominate this lineage, whereas TIR-NLRs and CC_R-NLRs are less expanded, with fewer than two copies per species on average. The TIR-NLR repertoire of Caryophyllales only includes the conserved TNL1 clade, which is also present in other plant phyla such as superasterids, superrosids, and magnolids. Furthermore, we found that NRG1, a well-known CC_R-NLR involved in immune signaling, is absent across all analyzed Caryophyllales species, while ADR1 remains present, suggesting a lineage-specific loss of NRG1. This comprehensive analysis of the Caryophyllales NLR repertoire provides a valuable resource for comparative genomics and resistance breeding in crops and wild species.