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Poster

Session: Session 2 Presenting Authors at Posters (Odds)

P-313 - Combining In Vitro and In Planta strategies to identify antagonistic bacteria against Candidatus Liberibacter asiaticus

Wednesday, July 16, 2025
13:30 - 15:15  
Location: Confex Hall 3
Plant microbiota functions and intermicrobial interactions

    Author(s)

  • AV

    Alessia Volpini

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • NM

    Nathalia Mancine

    Agronomic Institute - IAC / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • GP

    Giovana Peruchi

    Agronomic Intitute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • LM

    Lais Moura

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • HL

    Helena Lima

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

    • Presenting Author(s)

  • HC

    Helvecio Coletta-Filho

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

Among the citrus diseases, Huanglongbing (HLB), caused by the bacteria Candidatus Liberibacter asiaticus (CLas), remains to date the most damaging disease and difficult to control. The study of the disease is challenging because CLas is still unable to grow in culture. For that reason, microbes with genetic proximity to CLas, such as Liberibacter crescens and Sinorhizobium meliloti (SM), have been used in in vitro studies as surrogate organisms to identify chemical and biological antimicrobials against CLas. In the present work, we aimed to develop a trial system to optimize the search for antagonist microorganisms against CLas, combining an in vitro assay using SM as surrogate followed by CLas-infected in planta assay. Bacteria genera with previous information in the literature as antagonism to plant pathogens were selected in our citrus-rhizobacteria collection composed by 2880 16S rRNA sequenced individuals. So far, by in vitro screening, we have had positive results (inhibition halo against SM) for 11 different bacterial strains belonging to the genus Bacillus, Burkholderia, Chryseobacterium, Lelliottia, Methylobacterium, Serratia and Pseudomonas. Another 194 isolates belonging to these seven genera have been tested in vitro. In planta assays will follow using the most potential candidates previously selected in vitro assays.

 Supported by Fapesp: (2020/14584-8).