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Poster

Session: Session 1 Presenting Authors at Posters (Evens)

P-296 - Phytophthora infestans Effector Amplicon Sequencing (EASe)

Tuesday, July 15, 2025
09:55 - 11:30  
Location: Confex Hall 3
Population Biology, Ecology & Genomics

    Presenting Author(s)

  • SL

    Simeon Lim Rossmann, PhD (he/him/his)

    Tenure Track Postdoc
    Norwegian University of Life Sciences (NMBU)
    Ã…s, Akershus, Norway

    • Author(s)

  • EL

    Erik Lysøe

    Researcher
    NIBIO - Norwegian Institute for Bioeconomy Research
    Ã…s, Akershus, Norway

  • MS

    Monica Skogen

    Senior Engineer
    NIBIO - Norwegian Institute for Bioeconomy Research
    Ã…s, Akershus, Norway

  • HE

    HÃ¥vard Eikemo

    Researcher
    NIBIO - Norwegian Institute for Bioeconomy Research
    Ã…s, Akershus, Norway

  • MJ

    Marta Janiszewska

    PhD candidate
    Plant Breeding and Acclimatization Institute – National Research Institute, Platanowa
    Młochów, Mazowieckie, Poland

  • ML

    Mirella Ludwiczewska

    PhD student
    Plant Breeding and Acclimatization Institute – National Research Institute, Platanowa
    Młochów, Mazowieckie, Poland

  • SS

    Sylwester Sobkowiak

    Senior Engineer
    Plant Breeding and Acclimatization Institute – National Research Institute, Platanowa
    Młochów, Mazowieckie, Poland

  • JÅ

    Jadwiga Åšliwka

    Professor
    Plant Breeding and Acclimatization Institute – National Research Institute, Platanowa
    Młochów, Mazowieckie, Poland

  • MB

    May Bente Brurberg

    NIBIO - Norwegian Institute of Bioeconomy Research
    Ã…s, Akershus, Norway

Phytophthora infestans delivers effector proteins into the host’s apoplast and cells to manipulate defense responses and facilitate infection. Variations in  effector sequences and differences in their repertoire influence virulence and aggressiveness.

Isolates of P. infestans are routinely genotyped using neutral short sequence repeat (SSR) markers without assessment of effectors. We aimed to develop a complementary approach, Effector Amplicon Sequencing (EASe) to investigate whether this can add information on P. infestans populations and their virulence potential. This is particularly relevant for populations that are dominated by sexual reproduction. We derived effector sets comprising coding sequences for approximately 70 genes from 394 isolates from long read sequencing. We built an open-source, free, modular analysis pipeline for the EASe data and created a cohesive database of the distribution of variants for the investigated genes.

We found the expected strong conservation of effector sets within clonal lineages, with set differences ranging from 10 to 20 % within a genotype and averaging 35 % between random isolates. However, we also identified effector set types that were conserved between clonal lineages and likely sexually produced isolates, and types unique to groups of the latter.

EASe has the potential to improve the development of resistant potato cultivars and prediction of disease outcomes by matching effector gene variants and resistance genes.