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Poster

Session: Session 1 Presenting Authors at Posters (Evens)

P-334 - TET8 and two RNA helicases cooperatively regulate cross-kingdom RNAi and immunity to powdery mildew via membrane trafficking in barley

Tuesday, July 15, 2025
09:55 - 11:30  
Location: Confex Hall 3
RNA on the move: Advances in Cross-Species communiation &Translational Research

    Presenting Author(s)

  • QS

    Qian-Hua Shen

    Institute of Genetics and Developmental Biology, CAS
    Beijing, Beijing, China (People's Republic)

    • Author(s)

  • TD

    Tingting Du

    Institute of Genetics and Developmental Biology, CAS
    Beijing, Beijing, China (People's Republic)

  • LF

    Lei Feng

    Institute of Genetics and Developmental Biology, CAS
    Beijing, British Columbia, China (People's Republic)

  • YL

    Yanan Li

    Institute of Genetics and Developmental Biology, CAS
    Beijing, Beijing, China (People's Republic)

  • JL

    Jiali Li

    Institute of Genetics and Developmental Biology, CAS
    Beijing, Beijing, China (People's Republic)

  • ZC

    Ziyu Chen

    Institute of Genetics and Developmental Biology, CAS
    Beijing, Beijing, China (People's Republic)

  • XL

    Xue Li

    Institute of Genetics and Developmental Biology, CAS
    Beijing, Beijing, China (People's Republic)

  • YL

    Yaling Liu

    Institute of Genetics and Developmental Biology, CAS
    Beijing, Beijing, China (People's Republic)

  • YQ

    Yongli Qiao

    Shanghai Normal University
    Shanghai, Shanghai, China

  • HJ

    Huaibing Jin

    Institute of Plant Protection, CAAS
    Beijing, Beijing, China (People's Republic)

Cross-kingdom RNAi, a common strategy for regulating gene expression between hosts and their interacting pathogens, is often employed by plants to silence pathogen virulence genes for enhancing immunity. Here, we show that barley TETRASPANIN protein 8 (HvTET8) associates with extracellular vesicles (EVs) to transfer functional sRNAs into Blumeria graminis f. sp. hordei (Bgh), the powdery mildew fungal pathogen of barley. We generated barley transgenic lines overexpressing TET8-GFP fusion, and by confocal imaging observed dynamic membrane trafficking of HvTET8 that is focally accumulated underneath the attempted fungal penetration sites, localized to Bgh extrahaustorial membrane, and eventually moved into fungal haustorium. HvTET8 also associated with a pair of DEAD-box RNA helicases, HvRH37 and HvRH52 that could bind and deliver miRNAs into EVs and fungal haustorium, shown by imaging and biochemical analyses. Our data indicate that HvTET8 depends on HvRH37/52 to regulate cross-kingdom RNAi and immunity against Bgh fungus. Overall, our study reveals an in-depth molecular mechanism of TET8 and RH37/52 mediated vesicle trafficking and ck-RNAi in plant interaction with biotrophy fungal pathogen.