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Poster

Session: Session 1 Presenting Authors at Posters (Evens)

P-336 - Unlocking the role of small non-coding RNAs in Agrobacterium fabrum C58 extracellular vesicles: novel insights into inter-kingdom communication.

Tuesday, July 15, 2025
09:55 - 11:30  
Location: Confex Hall 3
RNA on the move: Advances in Cross-Species communiation &Translational Research

    Presenting Author(s)

  • FN

    Fanny Nazaret

    UMR CNRS 5557 Ecologie Microbienne, Université Claude Bernard Lyon 1
    Villeurbanne, Rhone-Alpes, France

    • Author(s)

  • TZ

    Timothée Zannis-Peyrot

    UMR 5557 Ecologie Microbienne, Université Claude Bernard Lyon 1
    Villeurbanne, Rhone-Alpes, France

  • DS

    Deniz Sarigol-Anis

    UMR 5557 Ecologie Microbienne, Université Claude Bernard Lyon 1
    Villeurbanne, Rhone-Alpes, France

  • CL

    Céline Lavire

    UMR 5557 Ecologie Microbienne, Université Claude Bernard Lyon 1
    Villeurbanne, Rhone-Alpes, France

  • LV

    Ludovic Vial

    UMR 5557 Ecologie Microbienne, Université Claude Bernard Lyon 1
    Villeurbanne, Rhone-Alpes, France

Bacterial extracellular vesicles (EVs) are powerful nanomessengers, carrying a diverse array of molecules - proteins, lipids, metabolites, and nucleic acids - and regarded as key players in intercellular communication. Among the transported cargos, small non-coding RNAs (sRNAs) have been identified in the EVs of several bacteria, and have drawn growing interest for their potential role in host-microorganism interactions. However, their involvement in plant-bacteria interactions remains largely unexplored.

In Agrobacterium fabrum C58, a rhizosphere bacterium capable of inducing root tumors via the transfer of its T-DNA, a sophisticated communication with the host plant relies on complex molecular exchanges. While A. fabrum EVs are likely to participate in these interactions, their precise role and the nature of their cargo remain to be elucidated. In particular, the presence of sRNAs in these vesicles raises the question of their involvement in the regulation of bacterial virulence or in the manipulation of plant responses.

In this context, our research aims to i) demonstrate the transfer of A. fabrum EVs to plants, ii) characterize their sRNAs content, and iii) identify candidates involved in virulence or the modulation of plant responses. This project will contribute to a better understanding of inter-kingdom communication mechanisms and the role of EVs in plant-bacterial interactions.