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Poster

Session: Session 1 Presenting Authors at Posters (Evens)

P-368 - Impact of symbiosis signalling in barley on interactions with beneficial and commensal soil bacteria

Tuesday, July 15, 2025
09:55 - 11:30  
Location: Confex Hall 3
Subcellular dynamics and signaling in host-microbe interactions
The Common Symbiosis Signalling Pathway (CSSP) is key to the formation of nitrogen-fixing and arbuscular mycorrhizal symbiosis (AMS) in plants, both vital for optimal nitrogen and phosphorus acquisition. While the role of CSSP genes in AMS is well-documented in cereals, their impact on other beneficial microbes and the overall assembly and function of the rhizosphere microbiome remains largely unknown. Understanding these interactions is crucial for overcoming nutrient limitations and developing organic fertilisers. Using barley CSSP mutants, we integrated microbiome profiling, metabolomics, and transcriptomics in soil and synthetic community settings to examine CSSP effects on bacterial assembly, identify CSSP-dependent root metabolites critical for plant-microbe signalling, and uncover additional genetic determinants involved in interactions with soil bacteria. Our results show that CSSP genes distinctly affect rhizosphere bacteria, including members of the Rhizobiales order, which hosts various nitrogen-fixing species. Root exudate analyses linked CSSP genes to the biosynthesis and release of signalling molecules such as coumarins, salicylic acid, auxin, and gibberellin. Furthermore, transcriptomic data revealed regulatory genes potentially mediating interactions with commensal and symbiotic bacteria. These findings enhance our understanding of cereal-microbe interactions and provide insights for engineering beneficial microbiomes to improve agricultural sustainability.