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Poster

Session: Session 1 Presenting Authors at Posters (Evens)

P-424 - Nitrate-induced NLP1 SUMOylation regulates nitrate signaling and root nodulation in Medicago truncatula

Tuesday, July 15, 2025
09:55 - 11:30  
Location: Confex Hall 3
Symbiosis and Mutualism (mycorrhiza and N-fixing Rhizobia)

    Presenting Author(s)

  • JL

    Jing Liu

    CAS Center for Excellence in Molecular Plant Sciences
    Shang Hai, Shanghai, China (People's Republic)

    • Author(s)

  • FX

    Fang Xie

    professor
    CAS Center for Excellence in Molecular Plant Sciences, Shanghai, China
    Shanghai, Shanghai, China (People's Republic)

Nitrate plays a vital role in plant growth and development, serving as both a critical signal and an essential nutrient. In leguminous plants, the symbiotic relationship with rhizobia for nitrogen acquisition is hindered by elevated external nitrate levels. MtNLP1 (NIN-like protein 1) plays a key role in the nitrate-induced suppression of nodulation. Yet, the precise mechanisms through which MtNLP1 regulates nodulation in the response to nitrate stimuli remain enigmatic. In this study, we unveil that nitrate stimulates the SUMO modification of MtNLP1. Substitution of three lysine residues  disrupts MtNLP1 SUMOylation, resulting in impaired functionality in the nitrate-induced suppression of nodulation and reduced interactions with MtNIN and MtNLP1. Additionally, we found that the components involved in SUMO modification in M. truncatula not only interact with each other but are also essential for symbiotic nitrogen fixation and the modulation of nodulation under nitrate influence. These discoveries reveal that SUMOylation plays a critical role in nitrate signaling transduction and the regulation of nitrate-mediated symbiotic nitrogen fixation.