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Poster

Session: Session 2 Presenting Authors at Posters (Evens)

P-158 - The conserved aphid saliva chemosensory protein effector Mp10 targets plant AMSH deubiquitinases at cellular membranes to suppress pattern-triggered immunity

Thursday, July 17, 2025
13:30 - 15:15  
Location: Confex Hall 3
Effector Interference Mechanisms

    Author(s)

  • MG

    Matteo Gravino

    Crop Genetics Dept., John Innes Centre,
    Norwich, England, United Kingdom

    • Presenting Author(s)

  • SM

    Sam T. Mugford

    John Innes Centre
    Norwich, England, United Kingdom

    • Author(s)

  • NK

    Nathan Kreuter

    Crop Genetics Dept., John Innes Centre
    Norwich, England, United Kingdom

  • JJ

    Joshua Joyce

    Crop Genetics Dept., John Innes Centre
    Norwich, England, United Kingdom

  • AK

    Adi Kliot

    Crop Genetics Dept., John Innes Centre
    Norwich, England, United Kingdom

  • JC

    James Canham

    The Sainsbury Laboratory
    Norwich, England, United Kingdom

  • CW

    Christine Wilson

    Crop Genetics Dept., John Innes Centre
    Norwich, England, United Kingdom

  • TM

    Thomas C. Mathers

    Crop Genetics Dept., John Innes Centre
    Norwich, England, United Kingdom

  • GS

    Gerhard Saalbach

    Proteomics Technology Platform, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, United Kingdom
    Norwich, England, United Kingdom

  • SH

    Saskia A. Hogenhout

    Group Leader
    Crop Genetics Dept., John Innes Centre
    Norwich, England, United Kingdom

Sap-feeding hemipteran insects such as aphids, whiteflies and leafhoppers use specialized mouthparts (stylets) to probe various plant cells and establish a long-term feeding site in the phloem. We previously demonstrated that aphids deposit the effector protein Mp10 into the cytoplasm of mesophyll cells. Mp10 (CSP4) belongs to a family of chemosensory proteins (CSPs) that are conserved in arthropods and have diverse functions. Here we investigate what processes CSPs regulate in plants. We found that Mp10 suppresses plant immunity in response to a variety of elicitor molecules, including aphid-derived elicitors. Mp10 interacts AMSH deubiquitinases, which are conserved plant target proteins with key roles in the trafficking and stabilization of membrane proteins, in agreement with our data showing that Mp10 inhibits ROS and Ca2+ bursts in response to flg22 Mp10 also promotes the degradation and cellular trafficking of the pattern recognition receptor FLS2. Phylogenetic analysis revealed that Mp10 belongs to a clade within the CSP family that include homologues from other plant-feeding hemipterans. Mp10 orthologues from other sap-feeders share PTI-suppressive functions and interactions with plant targets. Thus, CSPs in the Mp10 clade may have evolved to suppress PTI in plants early in the evolution of plant-feeding hemipterans. Since aphids deposit Mp10 into cells during probing, this effector may act as a local anesthetic, reducing the plant ability to detect aphid feeding.