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Poster

Session: Session 2 Presenting Authors at Posters (Evens)

P-148 - Rice ZIP3 regulates transcription of OsRBOHH through the interaction of bZIP transcription factor.

Thursday, July 17, 2025
13:30 - 15:15  
Location: Confex Hall 3
Effector Interference Mechanisms

Xanthomonas oryzae pv. oryzae (Xoo) causes a serious bacterial blight disease of rice. Previously we found that the transgenic rice plants expressing XopZ, one of the Xoo effectors, showed severe susceptibility to the type III secretion system-deficient mutant of Xoo.  Overexpression of XopZ suppressed PGN-induced ROS production, suggesting that XopZ may inhibit host factors involved in PAMPs-triggered resistance (PTI). To elucidate the molecular mechanisms of XopZ-mediated PTI inhibition, we tried to identify host targets that interact with XopZ by yeast two-hybrid screens and found three XopZ Interacting Proteins (ZIPs) as the host targets. Among them, ZIP3 is a hypothetical protein localized in nucleus and cytosol in rice. The knockout mutation of ZIP3 reduced resistance to Xoo and compromised PGN-induced ROS production. In addition, we found that ZIP3 interacted with bZIP transcriptional factor bZIPX. bZIPX positively regulated expression of OsRBOHH, which was suppressed by silencing of ZIP3. In this presentation, the molecular mechanism of ZIP3-mediated immunity in rice will be discussed.