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Poster

Session: Session 2 Presenting Authors at Posters (Evens)

P-234 - Silicate-solubilizing Burkholderia spp. confer tolerance of tomato to Fusarium oxysporum f. sp. lycopersici

Thursday, July 17, 2025
13:30 - 15:15  
Location: Confex Hall 3
Microbe-mediated impacts on plant-parasite interactions

    Author(s)

  • SK

    Sunil Khayamali

    PhD student
    International Doctoral Program in Agriculture, National Chung Hsing University, Taichung City 402202, Taiwan
    Taichung City, Taichung, Taiwan (Republic of China)

  • TC

    Tao-Ho Chang, PhD

    Assistant Professor
    Program in Plant Health Care, Academy of Circular Economy, National Chung Hsing University
    Nantou City, Nantou, Taiwan (Republic of China)

  • JH

    Jenn-Wen Huang, PhD

    Professor
    Program in Plant Health Care, Academy of Circular Economy, National Chung Hsing University, Nantou 540216, Taiwan; Department of Plant Pathology, National Chung Hsing University, Taichung City 402202, Taiwan
    Taichung City, Taichung, Taiwan (Republic of China)

    • Presenting Author(s)

  • PC

    Pi-Fang Linda Chang, PhD (she/her/hers)

    Professor
    Master Program in Plant Medicine and Good Agricultural Practice, National Chung Hsing University, Taichung City 402202, Taiwan; Program in Plant Health Care, Academy of Circular Economy, National Chung Hsing University, Nantou 540216, Taiwan; Department of Plant Pathology, National Chung Hsing University, Taichung City 402202, Taiwan
    Taichung City, Taichung, Taiwan (Republic of China)

Tomato cultivation has been challenged by Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (FOL). Tomato producers rely heavily on agrochemicals and resistant cultivars, but their effectiveness may decrease over time due to FOL diversity and evolution. Silicon, the second most abundant element in the earth’s crust, has been overlooked in crop protection due to its poor plant availability. Silicate-solubilizing bacteria (SSB) can transform inorganic silicates into a plant-available form, facilitating crop protection. This study isolated and identified thirteen rhizospheric SSBs through 16S rDNA sequencing. All the strains solubilized phosphate and produced indole-acetic acid, whereas only seven strains produced siderophores. Notably, three strains inhibited FOL, F. oxysporum f. sp. cubense, Magnaporthe oryzae, and Colletotrichum gloeosporioides in dual culture plate assay. Two best-performing strains, Burkholderia contaminans NRBC and B. aenigmatica NRBA, were selected for the greenhouse experiments, where they significantly suppressed disease incidence and progression without silicon supplementation. The expression of defense-related genes (PR1, ACS2, and ERF1) were relatively higher under NRBC treatment without silicon supplement, whereas WRKY70 had higher expression under silicon supplement at 8 days post-inoculation. Hence, both strains have the potential for biocontrol of FOL with the possible involvement of salicylic acid-mediated defense mechanism.