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Poster

Session: Session 2 Presenting Authors at Posters (Odds)

P-383 - The antagonistic effects of Bacillus subtilis and B. stercoris on the Heterodera schachtii and their influence on plant development

Wednesday, July 16, 2025
13:30 - 15:15  
Location: Confex Hall 3
Plant microbiota functions and intermicrobial interactions

    Presenting Author(s)

  • SA

    Seema Aslam

    Ph.D Scholar
    Univ of Bonn
    Bonn, Nordrhein-Westfalen, Germany

    • Author(s)

  • SB

    Sandra Bredenbruch

    Ph.D Scholar
    Univ of Bonn - Bonn
    Bonn, Nordrhein-Westfalen, Germany

  • FG

    Florian Grundler

    Professor
    Molecular Phytomedicine, University of Bonn
    Bonn, Nordrhein-Westfalen, Germany

  • SS

    Sylvia Schleker

    University of Bonn
    Bonn, Nordrhein-Westfalen, Germany

Plant-parasitic nematodes like H. schachtii reduce crop yields. Chemical control raises environmental concerns, highlighting the need for alternatives. PGPR are promising solution by suppressing nematodes while enhancing plant growth. This study investigated the potential of seven rhizobacteria isolates (SC11, SW33, SW44, SW53, SW69, SW73, and SW83) for H. schachtii suppression and plant growth promotion. In vitro assays showed that living cells (SW44, SW73) and cell-free supernatants (SW34) significantly increased J2 mortality (~64%). In vivo, A. thaliana trials confirmed reduced nematode infestation, with SW83 achieving the highest reduction (~69% females, ~58% total), and female size was also reduced by 12% (SC11) to 18% (SW44 and SW83) in treatments. Bacterial treatments also enhanced plant growth. Furthermore, we found that the bacteria significantly reduced the invasion of J2 into the plant roots compared to the control as observed 1, 2, and 3 dpi. 16S rDNA sequencing revealed that all seven rhizobacteria isolates belong to the genus Bacillus. Isolates SC11, SW33, and SW53 showed high homology to B. subtilis, while SW44, SW69, SW73, and SW83 were highly homologous to B. stercoris. This study is the first to report the antagonistic activity of B. stercoris against H. schachtii. Our investigation demonstrates that nematode-antagonistic bacteria can be a valuable means to be integrated into a nematode control strategy and additionally to enhance plant growth.