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Poster

Session: Session 2 Presenting Authors at Posters (Odds)

P-163 - The Ralstonia solanacearum PopP2 effector targets various transcriptional regulatory complexes in Arabidopsis

Wednesday, July 16, 2025
13:30 - 15:15  
Location: Confex Hall 3
Effector Interference Mechanisms
Gram-negative bacteria inject type III effectors into host cells to favour infection. Among those, effectors from the Yersinia outer protein J (YopJ) family of acetyltransferases, produced by diverse animal and plant bacterial pathogens, promote pathogen virulence by acetylating specific host components. However, the range of YopJ effectors’ targets remains elusive. Complementary approaches (yeast 2-hybrid and TurboID) allowed us to identify candidate Arabidopsis proteins targeted by the YopJ PopP2 effector from Ralstonia solanacearum. These candidates include three ARID (AT-Rich Interaction Domain) proteins, involved in the chromatin remodeling PEAT complexes and behaving as PopP2 substrates. Our data suggest that PopP2 modulate PEAT complexes assembly to promote Ralstonia infection in Arabidopsis. A proximity labelling assay using dexamethasone-inducible Arabidopsis lines expressing PopP2 fused with the Turbo biotin ligase revealed that PopP2 proxiome was significantly enriched in components of several chromatin remodeling complexes. Interestingly, the Xanthomonas campestris pv campestris XopJ6 YopJ effector, a very close homolog of PopP2, also interacts with PEAT components and contains in its proxiome some regulators of chromatin condensation that are shared with PopP2. Together, our data suggest that both effectors promote infection by interfering with various host functions, in particular transcriptional regulation through targeting of chromatin remodeling complexes.