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Poster

Session: Session 2 Presenting Authors at Posters (Odds)

P-391 - Unraveling cultivated and uncultivated citrus rhizosphere microbiota to establish synthetic communities of beneficial plant-associated bacteria

Wednesday, July 16, 2025
13:30 - 15:15  
Location: Confex Hall 3
Plant microbiota functions and intermicrobial interactions

    Author(s)

  • HL

    Helena Lima

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • NM

    Nathalia Mancine

    Agronomic Institute - IAC / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • LM

    Lais Moura

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • AV

    Alessia Volpini

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • GP

    Giovana Peruchi

    Agronomic Intitute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

    • Presenting Author(s)

  • HC

    Helvecio Coletta-Filho

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

Reconstructing the plant-associated microbiota has been explored as a strategy to promote sustainable agriculture as well particularly relevant for citrus seedling production, and plant growth in field. This study develops a synthetic microbial community (SynCom) to enhance citrus resilience. Rhizosphere soil from 12-year-old Valencia orange trees on Swingle citrumelo rootstock was collected across four São Paulo State (Brazil) regions with distinct climates and soils. The rhizosphere microbial diversity and the cultivable fraction of rhizobacteria were analyzed. A total of 2,880 bacterial isolates were recovered, belonging to 91 genera, representing 17% of the core rhizosphere microbiome. Of these, 136 isolates were screened for plant growth-promoting (PGP) traits in vitro, such as siderophore production, nitrogen fixation, indole-3-acetic acid (IAA) production, and phosphate solubilization. Growth enhancement for citrus seedlings was assessed via biomass accumulation and root morphology. All selected isolates exhibited at least one PGP trait, while 8 and 30 isolates significantly increased shoot and root biomass, respectively. Additionally, 107 and 92 isolates enhanced average root diameter and root volume, respectively. Based on these findings, three isolates were selected to assemble five distinct SynCom, which are currently being tested in vivo to assess their synergistic potential in promoting citrus seedling growth.

Supported by Fapesp: (2020/14584-8 and 2023/03131-0)