Poster
Yiheng Hu
Centre of Plant Molecular Biology (ZMBP), University of Tübingen
Tuebingen, Baden-Wurttemberg, Germany
Johanna Bode
Centre of Plant Molecular Biology (ZMBP), University of Tübingen
Tuebingen, Baden-Wurttemberg, Germany
Daniel Gómez-Pérez
Centre of Plant Molecular Biology (ZMBP), University of Tübingen
Tuebingen, Baden-Wurttemberg, Germany
Marco Guerreiro
Max-Planck Institute for Evolutionary Biology
Plön, Schleswig-Holstein, Germany
Alfredo Mari
Centre of Plant Molecular Biology (ZMBP), University of Tübingen
Tuebingen, Baden-Wurttemberg, Germany
Kun Wang
Centre of Plant Molecular Biology (ZMBP), University of Tübingen
Tuebingen, Baden-Wurttemberg, Germany
Sinja Niemann
Centre of Plant Molecular Biology (ZMBP), University of Tübingen
Tuebingen, Baden-Wurttemberg, Germany
Maryam Mahmoudi
Centre of Plant Molecular Biology (ZMBP), University of Tübingen
Tuebingen, Baden-Wurttemberg, Germany
Ariane Kemen
Centre of Plant Molecular Biology (ZMBP), University of Tübingen
Tuebingen, Baden-Wurttemberg, Germany
Paloma Durán
Max Planck Institute for Plant Breeding Research
Cologne, Nordrhein-Westfalen, Germany
Benjamin Schwessinger
Australian National University
Acton, Australian Capital Territory, Australia
Stephane Hacquard
MPIPZ
Cologne, GERMANY
Eva Stukenbrock
University of Kiel
Eric Kemen
Centre of Plant Molecular Biology (ZMBP), University of Tübingen
Tuebingen, Baden-Wurttemberg, Germany
The foliage of plants harbors a remarkably diverse and dynamic microbial community critical to plant health and ecosystem functions. Microbial interactions shape the trajectory of the leaf microbiome assembly, yet the mechanism governing this process remains elusive. In this study, we conducted a comprehensive microbiome survey across Europe to uncover interactions among hub microbes naturally occurring on Arabidopsis thaliana leaves. We elucidated the molecular mechanism of a mutualistic relationship between two hub taxa in the A. thaliana leaf microbiome: the yeast Dioszegia hungarica and the obligate oomycete parasite Albugo laibachii. Our results revealed that D. hungarica facilitates A. laibachii colonization by synthesizing and supplying thiamine, a vitamin that A. laibachii cannot produce. We also found evidence that natural selection has acted on the regulation of thiamine production of D. hungarica, thereby shaping this mutualistic interactions. In an ecological context, we showed this cross-feeding mechanism not only enhanced A. laibachii's colonization, but also benefited D. hungarica by promoting its longevity on the leaves, supporting the mutualism. Our study underscores the microbial cooperation in niche adaptation on plant leaves, providing important molecular and evolutionary insights into the ecological processes of establishment and stabilization of plant microbiomes.