Poster
Pichaporn Chuenban
1Heinrich Heine University Düsseldorf, Faculty of Mathematics and Natural Sciences, Centre for Plant Genome Engineering
Düsseldorf, Nordrhein-Westfalen, Germany
Katarzyna Makowska
1Heinrich Heine University Düsseldorf, Faculty of Mathematics and Natural Sciences, Centre for Plant Genome Engineering
Düsseldorf, Nordrhein-Westfalen, Germany
Susanne K. Vollmer
1Heinrich Heine University Düsseldorf, Faculty of Mathematics and Natural Sciences, Centre for Plant Genome Engineering, Düsseldorf, Germany 2Institute for Plant Sciences, University of Cologne, Cologne, Germany 3Cluster of Excellence in Plant Sciences, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Düsseldorf, Nordrhein-Westfalen, Germany
Maximilian Kreikamp
1Heinrich Heine University Düsseldorf, Faculty of Mathematics and Natural Sciences, Centre for Plant Genome Engineering
Düsseldorf, Nordrhein-Westfalen, Germany
Linda Schoppmann
1Heinrich Heine University Düsseldorf, Faculty of Mathematics and Natural Sciences, Centre for Plant Genome Engineering
Düsseldorf, Nordrhein-Westfalen, Germany
Markus Stetter
2Institute for Plant Sciences, University of Cologne, Cologne, Germany 3Cluster of Excellence in Plant Sciences, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Cologne, Nordrhein-Westfalen, Germany
Pouneh Pouramini
1Heinrich Heine University Düsseldorf, Faculty of Mathematics and Natural Sciences, Centre for Plant Genome Engineering
Düsseldorf, Nordrhein-Westfalen, Germany
Goetz Hensel
Head Centre for Plant Genome Engineering
1Heinrich Heine University Düsseldorf, Faculty of Mathematics and Natural Sciences, Centre for Plant Genome Engineering, Düsseldorf, Germany, 3Cluster of Excellence in Plant Sciences, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Düsseldorf, Nordrhein-Westfalen, Germany
The Centre for Plant Genome Engineering (CPGE) was established at Heinrich Heine University Düsseldorf in 2020. It is affiliated with the Cluster of Excellence in Plant Science (CEPLAS), which includes Heinrich Heine University, the University of Cologne, the Max Planck Institute for Plant Breeding Research, and the Research Centre Jülich. The CPGE provides CEPLAS scientists with expertise and services related to the genetic transformation of plants, targeted mutagenesis using customised endonucleases, and the development and implementation of expression systems. The CPGE employs transformation protocols based on Rhizobium radiobacter and biolistics. We utilise reporter genes encoding Green Fluorescent Protein or RUBY to establish transformation protocols or test expression systems. Barley, tobacco, Arabidopsis are routinely transformed, along with other species such as sunflower, amaranth, and lupin. We have established targeted mutagenesis in sunflower and grain amaranth. We have optimised two in vitro culture regeneration systems for obtaining transgenic or mutant plants: somatic embryogenesis and organogenesis. The methods and protocols we have developed facilitate the identification of molecular networks underlying chloroplast development and plant-microbe interactions while minimising photorespiration. A summary of CPGE activities will be provided.