Skip to main content
ISMPMI Congress 2025 Main banner
Icon Legend
This session is not in your schedule.
This session is in your schedule. Click again to remove it.

Poster

Session: Session 2 Presenting Authors at Posters (Evens)

P-312 - Citrus rootstock rhizobacteria community shaped by drought stress under field conditions

Thursday, July 17, 2025
13:30 - 15:15  
Location: Confex Hall 3
Plant microbiota functions and intermicrobial interactions

    Author(s)

  • GP

    Giovana Peruchi

    Agronomic Intitute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • NM

    Nathalia Mancine

    Agronomic Institute - IAC / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • AV

    Alessia Volpini

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • LM

    Lais Moura

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

  • HL

    Helena Lima

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

    • Presenting Author(s)

  • HC

    Helvecio Coletta-Filho

    Agronomic Institute (IAC) / Citrus Research Center (CCSM)
    Cordeiropolis, Sao Paulo, Brazil

Plant microbial communities contribute to drought tolerance by modulating phytohormones and producing biofilms. This study aims to characterize rhizobacteria populations in citrus rootstock (Swingle citrumelo) under variable drought stress. We hypothesize that plants with less stress harbor distinct rhizosphere communities compared to those with a severe stress phenotype. Root samples were collected during winter (dry) and summer (rainy) seasons in the northern region of São Paulo. Rhizobacteria were isolated using 4 culture media, yielding 4,682 isolates. The V4–V5 region of the 16S rRNA gene was sequenced using the Illumina MiSeq platform, and taxonomic classification was performed at the genus level using the amplicon sequence variant (ASV) strategy. Representatives from each genus were selected based on previous reports of plant growth promotion, resulting in 40 isolates (ASVs) from 22 genera. In vitro characterization included drought stress tests, amplification of ACC deaminase gene by PCR, and biofilm formation assays. The results showed that only three genera were shared between both sampling seasons. Bacteria from the dry season exhibited greater growth in drought stress tests and had higher ACC deaminase production compared to those from the rainy season. These findings enhance our understanding of plant-associated bacteria and their potential to mitigate water stress in citrus production.

Supported by Fapesp: (2020/14584-8 and 2023/13314-5)