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Poster

Session: Session 2 Presenting Authors at Posters (Evens)

P-242 - Distinct populations of bacterial extracellular vesicles from phytobacteria induce systemic changes in plant

Thursday, July 17, 2025
13:30 - 15:15  
Location: Confex Hall 3
Nutrient exchange & role of metabolites in plant-microbe interactions

    Presenting Author(s)

  • TZ

    Timothée Zannis-Peyrot

    UMR 5557 Ecologie Microbienne, Université Claude Bernard Lyon 1
    Villeurbanne, Rhone-Alpes, France

    • Author(s)

  • LD

    Lucas Degusseau

    UMR 5557 Ecologie Microbienne, Université Claude Bernard Lyon 1
    Villeurbanne, Rhone-Alpes, France

  • PD

    Pierre-Yves Dugas

    UMR 5128 CP2M, Université Claude Bernard Lyon 1, CPE Lyon
    Villeurbanne, Rhone-Alpes, France

  • FB

    Fabiola Bastian

    DTAMB FR BioEEnVis, Université Claude Bernard Lyon 1
    Villeurbane, Rhone-Alpes, France

  • MG

    Matthieu Gaucher

    IRHS-UMR1345, Université d’Angers, INRAE, Institut Agro, Angers, France
    Angers, Pays de la Loire, France

  • JD

    Jeanne Doré

    UMR 5557 Ecologie Microbienne, Université Claude Bernard Lyon 1
    Villeurbanne, Rhone-Alpes, France

  • VG

    Vincent Gaillard

    UMR 5557 Ecologie Microbienne, Université Claude Bernard Lyon 1
    Villeurbanne, Rhone-Alpes, France

  • FW

    Florence Wisniewski-Dyé

    UMR 5557 Ecologie Microbienne, Université Claude Bernard Lyon 1
    Villeurbanne, Rhone-Alpes, France

  • IK

    Isabelle Kerzaon

    UMR 5557 Ecologie Microbienne, Université Claude Bernard Lyon 1
    Villeurbanne, Rhone-Alpes, France

  • CL

    Céline Lavire

    UMR 5557 Ecologie Microbienne, Université Claude Bernard Lyon 1
    Villeurbanne, Rhone-Alpes, France

  • LV

    Ludovic Vial

    UMR 5557 Ecologie Microbienne, Université Claude Bernard Lyon 1
    Villeurbanne, Rhone-Alpes, France

Bacterial extracellular vesicles (BEVs) are lipidic shuttles that allow cells to export a diverse molecular cargo (proteins, lipids, nucleic acids and metabolites) enabling bacteria to interact with both microbial communities and host organisms. While they have primarily been investigated in animal-bacteria interactions, knowledge regarding phytobacterial BEVs remains limited. BEVs from phytobacteria can ellicitate immune responses and modulate the plant physiology in a complex manner. Notably, plant responses to BEVs can persist for days, enhancing the host’s ability to resist phytopathogen infections. We hypothetized that BEVs from phytobacteria would influence the plant defense gene expression and metablome at the systemic level. Furthermore, we proposed that phytobacteria can produce different BEVs populations in response to environmental cues, each exerting specific effects on plant physiology. We assessed the impact of BEVs derived from both beneficial and pathogenic bacteria on the tomato metabolome using LC-MS² analyses, as well as on defense gene expression throught RT-qPCR. Our results revealed that BEV exposure altered both local and systemic metabolite accumulation, particularly affecting hydroxycinnamic acids and steroidal alkaloids. Moreover, the plant response varied depending on the BEV population applied. These findings provide evidence of a global effect of BEVs on the plant and highlights the complex nature of plant-bacteria interactions mediated by BEVs.