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Poster

Session: Session 2 Presenting Authors at Posters (Evens)

P-036 - Exploiting NBS-LRR Microsatellite Variations for Basal Stem Rot Resistance Screening in Oil Palm Germplasm

Thursday, July 17, 2025
13:30 - 15:15  
Location: Confex Hall 3
Crop resistance genetics and genomics

    Presenting Author(s)

  • HT

    Hun Jiat Tung

    Senior Research Officer
    Advanced Agriecological Research Sdn Bhd
    Kota Damansara, Selangor, Malaysia

    • Author(s)

  • WW

    Wei Chee Wong

    Principal Research Officer
    Advanced Agriecological Research Sdn Bhd
    Petaling Jaya, Selangor, Malaysia

  • CW

    Choo Kien Wong

    Principal Research Officer
    Advanced Agriecological Research Sdn Bhd
    Petaling Jaya, Selangor, Malaysia

  • SC

    Shenyang Chin

    Advanced Agriecological Research Sdn Bhd
    Petaling Jaya, Selangor, Malaysia

Conventional screening for Ganoderma boninense resistance in oil palm (Elaeis guineensis Jacq.) is challenging in aspects of time, labour and space due to its perennial nature. Poor correlations between greenhouse screening and field observation further hampered the efforts to select resistant materials. To accelerate the process, marker-based screening is crucial. In this study, we investigated microsatellite variations in nucleotide-binding site leucine-rich repeat (NLR) genes within the oil palm genome using NLGenomeSweeper. The expression of 151 out of 213 predicted NLRs was validated through transcriptome data derived from naturally infected palms. The MIcroSAtellite Identification Tool was employed to detect the simple sequence repeats (SSRs) within the NLRs, and primers were designed to amplify these repeats. A total of 95 NLRs were found to contain SSRs, and only 25 sets of primers were successfully amplified in the intended region. Consequently, 19 polymorphic SSR markers were determined as suitable for genotyping oil palm germplasm comprising susceptible (Deli Dura) and partial resistant materials (Zaire x Cameroon). Results revealed the distinct clustering of susceptible and partially resistant palms, suggesting that the underexploited NLR microsatellite variations offer a novel tool for Ganoderma resistance screening. Further research is required to characterise NLR genes and their specificity towards Ganoderma infection.