P-038 - Exploring rice NLRs recognizing AVR effectors from non-host Magnaporthe pathogens

Thursday, July 17, 2025

13:30 - 15:15

Location: Confex Hall 3

Crop resistance genetics and genomics

Presenting Author(s)

ZM

Zin Mar Myint

Iwate Biotechnology Research Center
Yamanokami, Hanamaki City, Iwate, Japan

Author(s)

SA

Soichiro Asuke

Graduate School of Agricultural Science, Kobe University
Kobe, JAPAN
YT

Yukio Tosa

Graduate School of Agricultural Science, Kobe University
Kobe, Hyogo, Japan
RT

Ryohei Terauchi, PhD

Professor
Kyoto University
Muko, Kyoto, Kyoto, Japan
MS

Motoki Shimizu

Iwate Birotechnology Research Center
Kitakami, Iwate, Japan

A comprehensive survey of interactions between rice nucleotide-binding leucine-rich-repeat receptor proteins (NLRs) and avirulence effectors (AVRs) of the blast fungus Magnaporthe oryzae (syn. Pyricularia oryzae) is important for enhancing our understanding of plant-pathogen interactions. M. oryzae has a strict host specificity, which is determined by their AVR repertoire recognized by non-host plant species. To functionally explore rice NLRs, we selected M. oryzae pathotypes from various Poaceae species that are unable to infect rice due to their AVRs. We studied the genome sequences of Magnaporthe pathotypes and selected 161 candidate effector (eXO) genes that are present in Lolium, Eragrostis, Eleusine, Avena, Triticum and Brachiaria pathotypes, but absent from Oryza pathotype. These eXO genes were introduced into an isolate of Oryza pathotype to investigate their interaction with multiple rice cultivars. The result showed that some transformants with eXO genes induced resistance to a subset of rice varieties, indicating that these effectors are recognized as AVRs in rice. We are in the process of isolating cognate NLR genes by QTL-seq approach.