Poster
Sarah Marie Wolf
PhD student
MPIPZ
Cologne, Nordrhein-Westfalen, Germany
Johannes Ben Herpell
PostDoc
Max Planck Institute for Plant Breeding Research
Cologne, Nordrhein-Westfalen, Germany
Frederickson D. Entila
Post_Doctoral Researcher
MPIPZ
Cologne, Nordrhein-Westfalen, Germany
Pratchaya Pramoj Na Ayutthaya
PhD student
MPIPZ
Cologne, Nordrhein-Westfalen, Germany
Guillaume Chesneau
Max Planck Institute for plant breeding research
Cologne, Nordrhein-Westfalen, Germany
Silvina Perin
Max Planck Institute for Plant Breeding Research
Cologne, Nordrhein-Westfalen, Germany
Stephane Hacquard
MPIPZ
Cologne, GERMANY
Root-associated microbes produce a staggering diversity of exometabolites, some of which are expected to modulate host development. This research aims to identify and characterise metabolites derived from the plant root microbiota, which can elicit a developmental or immunological plant response. To do so, an Arabidopsis thaliana root-associated microbial selection of 94 bacteria was screened for an impact on seed germination. Bacteria were streaked individually on full-strength Murashige-Skoog-medium agar plates supplemented with 10 mM succinate, and Arabidopsis thaliana seeds were placed at different distances from the bacterial lawn. Our results indicate that 1) the majority of the tested microbes inhibited seed germination at close distances, 2) a few phylogenetically unrelated strains showed inhibition at larger distances, and 3) most of these effects were mediated by diffusible metabolites rather than by volatile organic compounds. The identification of seed germination-modulating bacterial exometabolites will advance the knowledge in seed perception of biotic environmental factors and their response to it.