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Poster

Session: Session 1 Presenting Authors at Posters (Odds)

P-177 - Identification of Single Nucleotide Polymorphisms (SNPs) responsible for virulence differences between Pseudomonas syringae pv. tabaci strain 6605 and 7375

Monday, July 14, 2025
13:30 - 15:15  
Location: Confex Hall 3
Microbial infection strategies (pathogens & non-pathogens)
Elucidating the differences in virulence of plant pathogenic bacteria holds the potential to enhance our understanding of infection mechanisms. Given the virulence arising from the intricate interplay of numerous genes, regulatory networks, and environmental factors, identifying the specific causative genes that differentiate virulence between strains remains challenging. Pseudomonas syringae pv. tabaci (Pta), a gram-negative bacterium responsible for wildfire disease in Nicotiana tabacum, serves as a valuable model. In our study, we conducted a comparative genomic analysis between two strains: the highly virulent Pta6605 and the less virulent Pta7375. Although these strains exhibit markedly different levels of virulence, they share a high degree of overall genomic similarity. Our investigation identified several single nucleotide polymorphisms (SNPs) in key open reading frames that result in premature stop codons, and we confirmed that two independent SNPs significantly influence the observed virulence differences. Locus substitution experiments further demonstrated that introducing specific SNPs from Pta7375 into Pta6605 leads to a notable reduction in virulence. This work not only elucidates the genetic factors that govern strain-specific virulence in Pta but also offers novel insights into the complex genetic interactions underlying bacterial virulence.