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Poster

Session: Session 1 Presenting Authors at Posters (Odds)

P-183 - Interactions between Inonotus obliquus mycelium and Betula platyphylla mesophyll protoplasts during co-culture

Monday, July 14, 2025
13:30 - 15:15  
Location: Confex Hall 3
Microbial infection strategies (pathogens & non-pathogens)

    Presenting Author(s)

  • RF

    Rizki Fitria

    Tokyo University of Agriculture and Technology, Utsunomiya University
    Utsunomiya, Japan, JAPAN

    • Author(s)

  • HS

    Hamako Sasamoto

    Tokyo University of Agriculture and Technology
    Utsunomiya, Tochigi, Japan

  • AW

    Ayaka Watanabe

    Utsunomiya University
    Utsunomiya, Tochigi, Japan

  • IN

    Ikumi Nezu

    Utsunomiya University
    Utsunomiya, Tochigi, Japan

  • FI

    Futoshi Ishiguri

    Utsunomiya University
    Utsunomiya, Tochigi, Japan

  • SY

    Shinso Yokota

    Utsunomiya University
    Utsunomiya, Tochigi, Japan

Inonotus obliquus, a Basidiomycota fungus, causes canker-rot disease in Japanese birch (Betula platyphylla Sukaczev var. japonica (Miq.) H. Hara). At the cell level, I. obliquus mycelial growth was promoted through co-culture with B. platyphylla mesophyll protoplasts, followed by the formation of yellow substance. However, the mechanisms underlying the interactions between I. obliquus mycelium and B. platyphylla protoplasts remain unclear. To address this, the interactions between mycelium and protoplasts were examined, focusing on the compounds responsible for the yellow substances and the glucosamine content after co-culture. In this study, the I. obliquus mycelium and B. platyphylla protoplasts were co-cultured in a 24-well plate for 40 days, and the growth index of mycelium and protoplasts, yellow substance formation, and glucosamine content were analyzed. The growth index of mycelium was higher in wells with protoplasts, indicating enhanced mycelial growth under co-culture conditions. However, protoplast growth was suppressed as mycelial concentrations increased. 1H-NMR data identified the yellow substance in the culture contained aliphatic compounds. The glucosamine content after co-culture and the interactions between I. obliquus mycelium and B. platyphylla protoplasts will be discussed in the presentation.