P-321 - Harnessing RNA mobility for targeted RNA-based crop protection against Syndrome Basses Richesses (SBR)

The sugar beet disease Syndrome Basses Richesses (SBR) is caused by the proteobacterium Candidatus Arsenophonus phytopathogenicus and the phytoplasma Candidatus Phytoplasma solani, transmitted by Pentastiridius leporinus. The spread of SBR results in significant reductions in sugar content and biomass, posing a major threat to sugar beet cultivation. Current management strategies, such as crop rotation, are disruptive and economically challenging, while conventional insecticides are no longer permitted. RNA interference (RNAi) offers a promising, concrete, and eco-friendly alternative for sustainable pest and pathogen control. Here, we present an RNA-based approach that integrates double-stranded RNA (dsRNA) and antisense circular RNAs (circRNAs) to selectively target multiple essential genes in both the insect vector and the bacterial pathogens. A key aspect of this strategy is the development of phloem-compatible RNA formulations to enable systemic uptake and effective transport within the plant. The research addresses fundamental aspects of RNA stability, uptake mechanisms, and delivery efficiency, which are crucial for optimizing the efficacy of RNA-based crop protection. This study highlights the potential of RNA-based plant protection strategies as a novel approach for controlling vector-borne plant diseases. The findings contribute to advancing molecular plant-microbe interaction research and underscore the role of RNAi as a powerful tool in sustainable agriculture.