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Poster

Session: Session 1 Presenting Authors at Posters (Odds)

P-385 - Remodelling of the Arabidopsis thaliana leaf RBPome in response to Pseudomonas syringae infection

Monday, July 14, 2025
13:30 - 15:15  
Location: Confex Hall 3
Subcellular dynamics and signaling in host-microbe interactions

RNA binding proteins (RBPs) are proteins that interact with RNA, modulate gene expression, and play a role in the growth, development, and defence responses of plants. Pathogens use multiple strategies to evade the plant’s immune system and establish infection including the use of effectors and other small molecules. Upon infection, the RBP landscape gets remodelled which results in the modulation of plant immunity. In this work, a technique known as plant RNA Interactome Capture (ptRIC) was used to identify Arabidopsis thaliana leaf RBPs that are differentially binding RNA during Pseudomonas syringae pv. tomato DC3000 (PsPto) infection. Infection assays were used to determine the immune phenotype of the A. thaliana mutants of the differential RBPs. The AI protein structure prediction tool Alphafold Multimer was used to predict if PsPto effectors were directly interacting with RBPs to alter their RNA binding activity. The characterisation of RBPs involved in plant immunity would help develop crops in the future that are more resistant to pathogens by exploiting RBPs and associated networks.