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Poster

Session: Session 1 Presenting Authors at Posters (Odds)

P-401 - Decoding AM symbiosis: Novel roles of RLCK and CBX1 in cytoplasmic and transcriptional regulation

Monday, July 14, 2025
13:30 - 15:15  
Location: Confex Hall 3
Symbiosis and Mutualism (mycorrhiza and N-fixing Rhizobia)

    Presenting Author(s)

  • ES

    Esperanza Abril Sosa-Lopez

    Institute for Plant Sciences, Cologne Biocenter, University of Cologne
    Cologne, Nordrhein-Westfalen, Germany

    • Author(s)

  • GS

    Georgios Saridis

    AG Döhlemann, Terrestrische Mikrobiologie
    Köln, Nordrhein-Westfalen, Germany

  • LX

    Li Xue

    Zhejiang Normal University
    Jinhua, Zhejiang, China (People's Republic)

  • LK

    Lompong Klinnawee

    Prince of Songkla University
    Songkla, Songkhla, Thailand

  • LW

    Leonie Weber

    Institute for Plant Sciences, Cologne Biocenter, University of Cologne
    Cologne, Nordrhein-Westfalen, Germany

  • MB

    Marcel Bucher

    Institute for Plant Sciences, Cologne Biocenter, University of Cologne
    Cologne, Nordrhein-Westfalen, Germany

The symbiosis between land plants and arbuscular mycorrhizal (AM) fungi is regulated by the Common Symbiosis Signaling Pathway (CSSP), which is evolutionarily conserved in all host plants, including Lotus japonicus. This network transmits signals from fungal recognition at the cell periphery to nuclear responses via calcium spiking. Key components include: SymRK, CYCLOPS and CCaMK. Despite significant progress in identifying players in the AM symbiosis, two critical aspects remain unclear: 1) the regulation of cytoplasmic signaling upstream of Ca2+ spiking, and 2) the transcriptional control of genes downstream of CYCLOPS-CCaMK. Using a combination of transcriptomics, reverse genetics and molecular biology, we identified two novel target genes of CYCLOPS-CCaMK: RLCK1, a plasma membrane-associated cytoplasmic kinase (RLCK), and the WRINKLED (WRI) transcription factor CBX1, a key regulator of mutual nutrient exchange during AM symbiosis. Our results provide new insights into the molecular regulation of AM symbiosis, in particular how RLCK mediates cytoplasmic signaling and how CBX1 contributes to transcriptional control in this mutualistic interaction.