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Poster

Session: Session 1 Presenting Authors at Posters (Evens)

P-058 - Molecular mechanisms of phosphatidic acid regulating PTI/ETI-mediated plant immunity

Tuesday, July 15, 2025
09:55 - 11:30  
Location: Confex Hall 3
Extracellular & Intracellular non-self recognition & signaling

    Presenting Author(s)

  • LK

    Liang Kong

    University of Michigan
    ANN ARBOR, Michigan, United States

    • Author(s)

  • XM

    Xiyu Ma

    Texas A&M University
    College Station, Texas, United States

  • CZ

    Chao Zhang

    University of Michigan
    ANN ARBOR, Michigan, United States

  • SK

    Sung-Il Kim

    University of Michigan
    ANN ARBOR, Michigan, United States

  • BL

    Bo Li

    Texas A&M University
    College Station, Texas, United States

  • YX

    Yingpeng Xie

    University of Michigan
    ANN ARBOR, Michigan, United States

  • IY

    In-Cheol Yeo

    University of Michigan
    ANN ARBOR, Michigan, United States

  • HT

    Hem Thapa

    Texas A&M University
    College Station, Texas, United States

  • SC

    Sixue Chen

    University of Mississippi
    Oxford, Mississippi, United States

  • TD

    Timothy P. Devarenne

    Texas A&M University
    College Station, Texas, United States

  • TM

    Teun Munnik

    University of Amsterdam
    Amsterdam, Noord-Holland, Netherlands

  • PH

    Ping He

    Professor
    University of Michigan
    Ann Arbor, MI, USA

  • LS

    Libo Shan

    Professor
    University of Michigan
    Ann Arbor, MI, USA

Phosphatidic acid (PA) and reactive oxygen species (ROS) are rapidly induced upon immune activation to limit pathogen colonization. However, how PA is intertwined with ROS signaling remains unclear. Here, we report that Arabidopsis diacylglycerol kinase 5 (DGK5) is essential for PTI- and ETI-induced PA production. The pattern recognition receptor (PRR)-associated kinase BIK1 phosphorylates DGK5 at Ser-506, triggering a rapid PA burst and activating plant immunity. To avoid excessive PA accumulation, the PRR-activated intracellular kinase MPK4 phosphorylates DGK5 at Thr-446, suppressing its activity and maintaining PA homeostasis. Similarly, ETI activation induces DGK5 phosphorylation and DGK5-derived PA production. Notably, PA binds to and stabilizes the NADPH oxidase RBOHD, thereby regulating ROS production in plant PTI and ETI, and their potentiation. Our findings reveal that distinct phosphorylation of DGK5 by PRR-activated BIK1 and MPK4 fine-tunes cellular PA homeostasis that regulates ROS generation in coordinating two branches of plant immunity.