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Poster

Session: Session 1 Presenting Authors at Posters (Evens)

P-078 - Roq1 receptor is more promiscuous than we used to think

Tuesday, July 15, 2025
09:55 - 11:30  
Location: Confex Hall 3
Extracellular & Intracellular non-self recognition & signaling

    Presenting Author(s)

  • MG

    Magdalena Górecka

    Institute of Biochemistry and Biophysics, Polish Academy of Sciences
    Warsaw

    • Author(s)

  • MJ

    Martyna Jonak

    Institute of Biochemistry and Biophysics, Polish Academy of Sciences
    Warsaw, Mazowieckie, Poland

  • MG

    Marta Grech-Baran

    PhD
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences
    Warsaw, POLAND

  • KS

    Kamil Steczkiewicz

    Institute of Biochemistry and Biophysics, Polish Academy of Sciences
    Warsaw, Mazowieckie, Poland

  • JO

    Juan Ochoa

    Institute of Biochemistry and Biophysics, Polish Academy of Sciences
    Warsaw, Mazowieckie, Poland

  • TK

    Tomasz Krepski

    Institute of Biochemistry and Biophysics, Polish Academy of Sciences
    Warsaw, Mazowieckie, Poland

  • PZ

    Patrycja Zembek

    Institute of Biochemistry and Biophysics, Polish Academy of Sciences
    Warsaw, Mazowieckie, Poland

  • JP

    Jaroslaw Poznanski

    Institute of Biochemistry and Biophysics, Polish Academy of Sciences
    Warsaw, Mazowieckie, Poland

  • KP

    Krzysztof Pawlowski

    Department of Molecular Biology, University of Texas, Southwestern Medical Center
    Dallas, Texas, United States

  • MK

    Magdalena Krzymowska

    Institute of Biochemistry and Biophysics, Polish Academy of Sciences
    Warsaw, POLAND

It is believed that improved crops protection against pathogens and pests may be achieved by stacking resistance-conferring genes such as Nucleotide–binding leucine rich receptors (NLRs) in plants. NLRs detect effectors either by direct association or by sensing their activity. Roq1 (Recognition of XopQ), NLR from Nicotiana genus, belongs to the TNL class (TIR domain-containing NLRs) and binds XopQ/HopQ1. By employing an array of pathoassays and protein-protein interactions analyses we showed that Roq1 directly binds also a Pseudomonas syringae type three effector from a divergent family. Using structural simulations we proposed a model of this interaction. Moreover, we analyzed the co-occurrence of effectors recognized by Roq1 in various phytopathogenic Pseudomonas strains.  Most strains rarely carry both effectors concurrently. This general tendency was observed for all identified phylogroups except for a phylogroup that encompasses strains originated from monocots and coffee plants, which do not possess or have strikingly diminished rate of TNLs, respectively. Our data indicate that plants evolved TNLs that exhibit overlapping ligand specificity revealing a neglected aspect of NLRs operating in a direct manner.