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Poster

Session: Session 1 Presenting Authors at Posters (Evens)

P-094 - The single CrRLK1L in the liverwort Marchantia polymorpha is a negative regulator of immunity

Tuesday, July 15, 2025
09:55 - 11:30  
Location: Confex Hall 3
Extracellular & Intracellular non-self recognition & signaling

    Presenting Author(s)

  • RS

    Raphael Schaal (he/him/his)

    Center for Plant Molecular Biology (ZMBP), University of Tuebingen
    Tübingen, DE, GERMANY

    • Author(s)

  • KS

    Kalina Stefanova

    Center for Plant Molecular Biology (ZMBP), University of Tuebingen
    Tuebingen, Baden-Wurttemberg, Germany

  • AV

    Adrián Vojtaššák

    Center for Plant Molecular Biology (ZMBP), University of Tuebingen
    Tuebingen, Baden-Wurttemberg, Germany

  • UH

    Ulrike Herzog

    Center for Plant Molecular Biology (ZMBP), University of Tuebingen
    Tuebingen, Baden-Wurttemberg, Germany

  • AG

    Alejandro Giraldo-Fonseca

    Department of Plant and Microbial Biology, University of Zurich
    Zurich, Zurich, Switzerland

  • ND

    Nicholas J. Desnoyer

    Department of Plant and Microbial Biology, University of Zurich
    Zurich, Zurich, Switzerland

  • MM

    Martin A. Mecchia

    Department of Plant and Microbial Biology, University of Zurich
    Zurich, Zurich, Switzerland

  • UG

    Ueli Grossniklaus

    Prof. Group Leader
    Department of Plant and Microbial Biology, University of Zurich
    Zurich, Zurich, Switzerland

  • CZ

    Cyril Zipfel

    Institute of Plant and Microbial Biology, University of Zurich / The Sainsbury Laboratory
    Zurich, Zurich, Switzerland

  • IM

    Isabel Monte

    Prof. Group Leader
    Center for Plant Molecular Biology (ZMBP), University of Tuebingen, University of Tuebingen
    Tuebingen, Baden-Wurttemberg, Germany

The Catharanthus roseus receptor-like kinase 1-like (CrRLK1L) family contains 17 members in Arabidopsis thaliana. CrRLK1Ls bind RAPID ALKALINIZATION FACTORs (RALFs) and regulate growth, reproduction and stress responses, including immunity. Notably, the CrRLK1Ls FERONIA and ANXUR1/ANXUR2 play positive and negative roles in regulation of pattern-triggered immunity (PTI), respectively. The liverwort Marchantia polymorpha is widely used for comparative evolutionary studies due to its phylogenetic position within the bryophytes and possesses a single CrRLK1L ortholog and only three RALFs. MpCrRLK1L (also referred to as MpTHE1/MpFER) has been shown to control cell elongation and cell wall integrity, but its involvement in the regulation of immunity in M. polymorpha remains unclear. Combining genetic and transcriptomic analyses with immune assays, we discovered that the Mpcrlk1l mutant and Mpralf double mutants show hyperactivation of immune-responsive genes and increased resistance to bacterial and fungal pathogens, consistent with their autoimmune phenotype. Structural modeling of MpCrRLK1L and the AtFER and AtANX crystal structures indicate that MpCrRLK1L is most similar to AtANX2, in line with such negative function in immunity. Complementation tests in Arabidopsis revealed that the CrRLK1L/RALF module plays conserved roles in land plants.