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Poster

Session: Session 1 Presenting Authors at Posters (Odds)

P-087 - Successful N-terminal tagging of the Pattern Recognition Receptor LORE

Monday, July 14, 2025
13:30 - 15:15  
Location: Confex Hall 3
Extracellular & Intracellular non-self recognition & signaling

    Presenting Author(s)

  • BS

    Bruno Smet

    Department of Biology, University of Fribourg
    Fribourg, Fribourg, Switzerland

    • Author(s)

  • FY

    Fan-Yu Yu

    PhD Student
    Department of Biology, University of Fribourg
    Fribourg, Fribourg, Switzerland

  • OT

    Olga Thiry

    Department of Biology, University of Fribourg
    Fribourg, Fribourg, Switzerland

  • MS

    Milena Schäffer

    Chair of Phytopathology, Technical University of Munich
    Munich, Bayern, Germany

  • SE

    Sabine Eschrig

    Chair of Phytopathology, Technical University of Munich, Germany
    Munich, Bayern, Germany

  • SR

    Stefanie Ranf

    Professor
    University of Fribourg
    Fribourg, Fribourg, Switzerland

Pattern Recognition Receptors (PRRs) are receptor kinases that detect Microbe-Associated Molecular Patterns (MAMPs) from invading microbes to activate Pattern-Triggered Immunity (PTI). The PRR LORE (LIPO-OLIGOSACCHARIDE-SPECIFIC REDUCED ELICITATION / SD1-29) recognises bacteria-derived medium chain 3-hydroxy fatty acids (3-OH-FA) in Brassicaceae to initiate PTI. Before, we characterised the importance of LORE homomerization in the activation of downstream signalling and the natural variation in 3-OH-FA perception in Brassicaceae. We now aim to uncover the details of early LORE mediated signalling. To this end, we have developed epitope-tagged versions of LORE to facilitate the identification of key post-translational modification sites and interaction partners. However, C-terminally tagged versions of LORE show altered signalling kinetics. We are therefore establishing a series of N-terminally tagged LORE transgenic lines, which appear to show wild-type short- and long-term responses to 3-OH-FA. Our approach now allows researchers to study the intracellular domain of receptor kinases in their native state, while still making use of many of the benefits that epitope tags provide.