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Poster

Session: Session 1 Presenting Authors at Posters (Odds)

P-153 - De-nitrosylation Coordinates Appressorium Function for Infection of the Rice Blast Fungus

Monday, July 14, 2025
13:30 - 15:15  
Location: Confex Hall 3
Microbial infection strategies (pathogens & non-pathogens)

    Presenting Author(s)

  • HH

    Hong Hu

    Huazhong Agricultural University College of Plant Science and Technology
    Wuhan, No.1, Shizishan, CHINA

    • Author(s)

  • WH

    Wenhui He

    Huazhong Agricultural University College of Plant Science and Technology
    Wuhan, Hubei, China (People's Republic)

  • ZQ

    Zhiguang Qu

    Huazhong Agricultural University College of Plant Science and Technology
    Wuhan, Hubei, China (People's Republic)

As a signaling molecule, nitric oxide (NO) regulates the development and stress response in different organisms. The major biological activity of NO is protein S-nitrosylation, whose function in fungi remains largely unclear. Here, it is found in the rice blast fungus Magnaporthe oryzae, de-nitrosylation process is essential for functional appressorium formation during infection. Nitrosative stress caused by excessive accumulation of NO is harmful for fungal infection. While the S-nitrosoglutathione reductase GSNOR-mediated de-nitrosylation removes excess NO toxicity during appressorium formation

to promote infection. Through an indoTMT switch labeling proteomics technique, 741 S-nitrosylation sites in 483 proteins are identified. Key appressorial proteins, such as Mgb1, MagB, Sps1, Cdc42, and septins, are activated by GSNOR through de-nitrosylation. Removing S-nitrosylation sites of above proteins is essential for proper protein structure and appressorial

function. Therefore, GSNOR-mediated de-nitrosylation is an essential regulator for appressorium formation. It is also shown that breaking NO homeostasis by NO donors, NO scavengers, as well as chemical inhibitor of GSNOR, shall be effective methods for fungal disease control.