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Poster

Session: Session 2 Presenting Authors at Posters (Odds)

P-449 - Spatiotemporally resolved organelle dynamics in resistosome-mediated hypersensitive cell death

Wednesday, July 16, 2025
13:30 - 15:15  
Location: Confex Hall 3
Spatial & temporal resolution of the interaction interface

    Presenting Author(s)

  • YC

    Yi-Feng Chen

    Academia Sinica
    Taipei, Nankang, TAIWAN

    • Author(s)

  • KL

    Kuan-Yu Lin

    Institute of Plant and Microbial Biology, Academia Sinica
    Taipei, Taipei, Taiwan (Republic of China)

  • CH

    Ching-Yi Huang

    Max Plank Institute for Biology Tübingen
    Tübingen, Baden-Wurttemberg, Germany

  • LH

    Liang-Yu Hou, PhD (he/him/his)

    Postdoctoral researcher
    Academia Sinica
    Taipei, Taipei, Taiwan (Republic of China)

  • CC

    Chin-Wen Chang

    Academia Sinica
    Taipei, Taipei, Taiwan (Republic of China)

  • HW

    Hung-Yu Wang

    Academia Sinica
    Taipei, Taipei, Taiwan (Republic of China)

  • TB

    Tolga Bozkurt, PhD (he/him/his)

    Reader
    Imperial College London
    London, England, United Kingdom

  • CW

    Chih-Hang Wu

    Associate Research Fellow
    Institute of Plant and Microbial Biology, Academia Sinica
    Taipei City, Taipei, Taiwan (Republic of China)

Abstract Text:

Plant nucleotide-binding domain leucine-rich repeat (NLR) proteins function as immune receptors that recognize pathogens and confer resistance. Upon activation, many NLRs assemble into membrane-associated resistosomes that trigger immune signaling and lead to hypersensitive cell death. However, the sequential subcellular events that connect resistosome activation to cell death remain unclear. In this study, we explored the spatiotemporal dynamics of organelles upon activation of NLR required for cell death 4 (NRC4) resistosome. Using a copper-inducible system, we performed time-lapse imaging after triggering NRC4-mediated hypersensitive cell death. By employing various subcellular markers, we observed that cytoplasmic streaming and organelle movement were disrupted before visible resistosome puncta appeared. Coinciding with the emergence of resistosome puncta, both actin and microtubules were depolymerized. This was followed by a loss of plasma membrane (PM) integrity, fragmentation of the endoplasmic reticulum into vesicle-like structures, and nuclear envelope breakdown. Eventually, the cells collapsed, with the PM and tonoplast shrinking toward the cell center. This study provides a precise temporal sequence of subcellular dynamics during plant hypersensitive cell death and lays the foundation for future research on the molecular mechanisms underlying subcellular processes following immune activation.