Spray-induced gene silencing (SIGS) is an emerging biocontrol technology that relies on the topical application of double-stranded RNA (dsRNA) to silence target genes in pests via RNA interference (RNAi). This approach has proven to be a promising next-generation pesticide, with demonstrated efficacy against various pests. Notably, Calantha™, the world’s first commercial SIGS product, was developed by GreenLight Biosciences to target the Colorado potato beetle. Calantha™ was approved for use by the EPA in December 2023 and is commercially available. Here, we present a comprehensive pipeline for developing RNAi fungicides and highlight recent advancements in SIGS technology, including its mode of action. A noteworthy example is unecyna, an RNAi-based, dsRNA active ingredient for a potential foliar-applied biofungicide that is designed to manage Erysiphe necator, the pathogen causing grape powdery mildew. Our results indicate that unecyna significantly reduces disease severity, emphasizing the potential of SIGS as a commercially viable solution for powdery mildew management. In addition, our studies have provided a deeper understanding of the SIGS mechanism, offering valuable insights for developing more effective RNAi-based fungicides. Overall, our findings underscore the value of SIGS in delivering effective and eco-friendly crop protection, while meeting the growing demand for sustainable agriculture.
