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Concurrent Session

Session: Concurrent Session: RNA on the move: Advances in Cross-Species Communication & Translational Research

RNA-Based Control of Downy Mildew Pathogens in Plants

Tuesday, July 15, 2025
11:30 - 11:50  
Location: Room P+Q

Downy mildew (DM) is a devastating disease that severely reduces the yield and quality of horticultural and other economically important crops, particularly in cool, humid conditions. Caused by obligate, host-specific oomycete pathogens, DM is challenging to control due to persistent spores and rapid mutation. While chemical pesticides have been the primary control strategy, their environmental risks remain a global concern. Our research utilizes RNA interference (RNAi) to dissect gene function, identify novel control targets, and explore host-DM interactions. We employed short-synthesized dsRNAs (SS-dsRNAs) to target numerous genes across three phylogenetically distinct DM pathogens—Hyaloperonospora arabidopsidis, Peronospora viciae f. sp. pisi, and Bremia lactucae. By comparing synthetic, in vitro-transcribed, and E. coli-produced dsRNAs, we optimized dsRNA length, concentration, and delivery methods to enhance gene silencing efficiency. We demonstrated multiplex silencing by targeting multiple genes simultaneously, revealing synergistic effects on pathogen suppression. Confocal microscopy confirmed dsRNA uptake by DM spores and germ tubes. This work establishes a scalable framework for RNAi-based functional studies in obligate oomycetes and provides insights for RNA-based biocontrol. Our findings also highlight the potential of multiplexed RNAi strategies for precision agriculture, offering an sustainable alternative to traditional fungicides for managing multiple DM pathogens.